Cloning and comparative mapping of recently evolved human chromosome 22-specific alpha satellite DNA

Mariano Rocchi, Nicoletta Archidiacono, Rachele Antonacci, Palma Finelli, Leonardo D'Aiuto, Roberta Carbone, Elizabeth Lindsay, Antonio Baldini

Research output: Contribution to journalArticle

Abstract

We have isolated and characterized a new alphoid probe, named p190.22. Its chromosomal location was investigated using fluorescence in situ hybridization. Under high stringency conditions p190.22 recognizes specifically the centromere of chromosome 22. A chromosome 22-specific alphoid subset has been previously reported in the literature (p22/1:2.1). The partial sequence and the genomic organization comparison strongly suggests that they recognize distinct subsets both specific for chromosome 22. The comparative mapping of probes p190.22 and p22/1:2.1 on chimpanzee (PTR and PPA) and gorilla (GGO) chromosomes was investigated. The two probes showed different hybridization results. p190.22, in particular, did not show any hybridization signal in these three species, suggesting a recent evolution.

Original languageEnglish
Pages (from-to)443-448
Number of pages6
JournalSomatic Cell and Molecular Genetics
Volume20
Issue number5
DOIs
Publication statusPublished - Sep 1994

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Rocchi, M., Archidiacono, N., Antonacci, R., Finelli, P., D'Aiuto, L., Carbone, R., Lindsay, E., & Baldini, A. (1994). Cloning and comparative mapping of recently evolved human chromosome 22-specific alpha satellite DNA. Somatic Cell and Molecular Genetics, 20(5), 443-448. https://doi.org/10.1007/BF02257462