Cloning and developmental expression of amphioxus Dachschund

Simona Candiani, Jana Kreslova, Vladimir Benes, Diana Oliveri, Patrizio Castagnola, Mario Pestarino, Zbynek Kozmik

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The nuclear factor dachshund (dac) is a key regulator of eye and leg development in Drosophila.We have cloned a Dachshund homologue from an invertebrate chordate amphioxus (Branchiostoma floridae). Sequence comparison reveals a high degree of similarity of amphioxus Dachshund (AmphiDach) to the known vertebrate and Drosophila dachshund genes. AmphiDach is first expressed in the prospective paraxial mesoderm at the gastrula stage. At the early neurula stage, expression is detected in developing somites and anterior endoderm, but in late neurula transcripts are present exclusively in the most posterior region of the cerebral vesicle and the anterior pharynx endoderm. Then, in larva, AmphiDach is localized in photoreceptive neurons of the frontal eye, infundibular organ, and endostyle as well as in Hesse organs and in nerve cells scattered along the nerve cord. Comparison of Dach expression in amphioxus and vertebrates suggests that such patterns are relatively similar (because they are expressed in somites, photoreceptor cells and CNS), even if expression of AmphiDach in the endostyle has no counterparts in its vertebrate homolog, the thyroid.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalGene Expression Patterns
Volume3
Issue number1
DOIs
Publication statusPublished - Mar 2003

Fingerprint

Lancelets
Organism Cloning
Vertebrates
Somites
Endoderm
Drosophila
Nonvertebrate Chordata
Neurons
Gastrula
Photoreceptor Cells
Mesoderm
Pharynx
Larva
Leg
Thyroid Gland

Keywords

  • Amphioxus
  • Branchiostoma floridae
  • Dachshund
  • Developmental expression
  • Eya
  • Eye
  • Pax
  • Six
  • Somites
  • Transcription factor

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Candiani, S., Kreslova, J., Benes, V., Oliveri, D., Castagnola, P., Pestarino, M., & Kozmik, Z. (2003). Cloning and developmental expression of amphioxus Dachschund. Gene Expression Patterns, 3(1), 65-69. https://doi.org/10.1016/S1567-133X(02)00070-4

Cloning and developmental expression of amphioxus Dachschund. / Candiani, Simona; Kreslova, Jana; Benes, Vladimir; Oliveri, Diana; Castagnola, Patrizio; Pestarino, Mario; Kozmik, Zbynek.

In: Gene Expression Patterns, Vol. 3, No. 1, 03.2003, p. 65-69.

Research output: Contribution to journalArticle

Candiani, S, Kreslova, J, Benes, V, Oliveri, D, Castagnola, P, Pestarino, M & Kozmik, Z 2003, 'Cloning and developmental expression of amphioxus Dachschund', Gene Expression Patterns, vol. 3, no. 1, pp. 65-69. https://doi.org/10.1016/S1567-133X(02)00070-4
Candiani, Simona ; Kreslova, Jana ; Benes, Vladimir ; Oliveri, Diana ; Castagnola, Patrizio ; Pestarino, Mario ; Kozmik, Zbynek. / Cloning and developmental expression of amphioxus Dachschund. In: Gene Expression Patterns. 2003 ; Vol. 3, No. 1. pp. 65-69.
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