Cloning and expression of two different genes from Streptococcus dysgalactiae encoding fibronectin receptors

Per Eric Lindgren, Pietro Speziale, Martin McGavin, Hans Jürg Monstein, Magnus Höök, Livia Visai, Timo Kostiainen, Silvia Bozzini, Martin Lindberg

Research output: Contribution to journalArticle

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Abstract

Binding of bacteria to fibronectin has been implicated as a mechanism of bacterial adhesion to the host tissue. In this report we have analyzed the binding of a strain of Streptococcus dysgalactiae to fibronectin. The cells bind to a site in the NH2-terminal domain of the protein via trypsin-sensitive cell surface components. Furthermore, a lysate prepared by sonication of streptococcal cells contained fibronectin-binding proteins that inhibit the binding of the ligand to intact bacteria. When the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted to an Immobilon-P filter, and probed with 125I-labeled fibronectin, a 140-kDa fibronectin-binding protein was identified along with a number of smaller binding proteins. A genomic DNA library was constructed and screened for the expression of fibronectin-binding proteins. Two clones were isolated and shown to contain unrelated inserts by restriction mapping and cross-hybridization experiments. The two encoded proteins were also immunologically distinct although both bound to the same region of the fibronectin molecule, and both effectively inhibited the binding of 125I-fibronectin to bacterial cells. Immunological analyses showed that only one of the two proteins tentatively identified as fibronectin receptors was expressed in detectable quantities in the Streptococcus dysgalactiae strain under the culture conditions employed.

Original languageEnglish
Pages (from-to)1924-1931
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number3
Publication statusPublished - Jan 25 1992

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Fibronectin Receptors
Cloning
Streptococcus
Fibronectins
Organism Cloning
Genes
Carrier Proteins
Bacteria
Proteins
Bacterial Adhesion
Restriction Mapping
Sonication
Genomic Library
Cellular Structures
Electrophoresis
Gene Library
Protein Binding
Sodium Dodecyl Sulfate
Trypsin
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lindgren, P. E., Speziale, P., McGavin, M., Monstein, H. J., Höök, M., Visai, L., ... Lindberg, M. (1992). Cloning and expression of two different genes from Streptococcus dysgalactiae encoding fibronectin receptors. Journal of Biological Chemistry, 267(3), 1924-1931.

Cloning and expression of two different genes from Streptococcus dysgalactiae encoding fibronectin receptors. / Lindgren, Per Eric; Speziale, Pietro; McGavin, Martin; Monstein, Hans Jürg; Höök, Magnus; Visai, Livia; Kostiainen, Timo; Bozzini, Silvia; Lindberg, Martin.

In: Journal of Biological Chemistry, Vol. 267, No. 3, 25.01.1992, p. 1924-1931.

Research output: Contribution to journalArticle

Lindgren, PE, Speziale, P, McGavin, M, Monstein, HJ, Höök, M, Visai, L, Kostiainen, T, Bozzini, S & Lindberg, M 1992, 'Cloning and expression of two different genes from Streptococcus dysgalactiae encoding fibronectin receptors', Journal of Biological Chemistry, vol. 267, no. 3, pp. 1924-1931.
Lindgren, Per Eric ; Speziale, Pietro ; McGavin, Martin ; Monstein, Hans Jürg ; Höök, Magnus ; Visai, Livia ; Kostiainen, Timo ; Bozzini, Silvia ; Lindberg, Martin. / Cloning and expression of two different genes from Streptococcus dysgalactiae encoding fibronectin receptors. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 3. pp. 1924-1931.
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