Cloning and gene mapping of the chromosome 13q14 region deleted in chronic lymphocytic leukemia

S. Kalachikov, A. Migliazza, E. Cayanis, N. S. Fracchiolla, M. F. Bonaldo, L. Lawton, P. Jelenc, X. Ye, X. Qu, M. Chien, R. Hauptschein, G. Gaidano, U. Vitolo, G. Saglio, L. Resegotti, V. Brodjansky, N. Yankovsky, P. Zhang, M. B. Soares, J. RussoI. S. Edelman, A. Efstratiadis, R. Dalla-Favera, S. G. Fischer

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Abstract

Frequent deletions and loss of heterozygosity in a segment of chromosome 13 (13q14) in cases of B-cell chronic lymphocytic leukemia (CLL) have suggested that this malignancy is caused by inactivation of an unknown tumor suppressor gene located in this region. Toward the identification of the putative CLL tumor suppressor, we have constructed a high-resolution physical map of YAC, PAC, and cosmid contigs covering 600 kb of the 13q14 genomic region. In addition to densely positioned genetic markers and STSs, this map was further annotated by localization of 32 transcribed sequences (ESTs) using a combination of exon trapping, direct cDNA selection, sample sequencing of cosmids and PACs, and homology searches. On the basis of these mapping data, allelic loss analyses at 13q14 using CLL tumor samples allowed narrowing of the genomic segment encompassing the putative CLL gene to

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalGenomics
Volume42
Issue number3
DOIs
Publication statusPublished - Jun 15 1997

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ASJC Scopus subject areas

  • Genetics

Cite this

Kalachikov, S., Migliazza, A., Cayanis, E., Fracchiolla, N. S., Bonaldo, M. F., Lawton, L., Jelenc, P., Ye, X., Qu, X., Chien, M., Hauptschein, R., Gaidano, G., Vitolo, U., Saglio, G., Resegotti, L., Brodjansky, V., Yankovsky, N., Zhang, P., Soares, M. B., ... Fischer, S. G. (1997). Cloning and gene mapping of the chromosome 13q14 region deleted in chronic lymphocytic leukemia. Genomics, 42(3), 369-377. https://doi.org/10.1006/geno.1997.4747