Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: Phylogenetic implications

Anna Maria Sanangelantoni, Daniela Barbarini, Giuseppe Di Pasquale, Piero Cammarano, Orsola Tiboni

Research output: Contribution to journalArticlepeer-review

Abstract

The glnA gene of the thermophilic sulphur-dependent archaebacterium Sulfolobus solfataricus was identified by hybridization with the corresponding gene of the cyanobacterium Spirulina platensis and cloned in Escherichia coli. The nucleotide sequence of the 1696 bp DNA fragment containing the structural gene for glutamine synthetase was determined, and the derived amino acid sequence (471 residues) was compared to the sequences of glutamine synthetases from eubacteria and eukaryotes. The homology between the archaebacterial and the eubacterial enzymes is higher (42%-49%) than that found with the eukaryotic counterpart (less than 20%). This was true also when the five most conserved regions, which it is possible to identify in both eubacterial and eukaryotic glutamine synthetases, were analysed.

Original languageEnglish
Pages (from-to)187-194
Number of pages8
JournalMGG Molecular & General Genetics
Volume221
Issue number2
DOIs
Publication statusPublished - Apr 1990

Keywords

  • Archaebacteria
  • Glutamine synthetase
  • Nuxleotide sequence
  • Phylogeny

ASJC Scopus subject areas

  • Genetics

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