TY - JOUR
T1 - Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene
T2 - Phylogenetic implications
AU - Sanangelantoni, Anna Maria
AU - Barbarini, Daniela
AU - Di Pasquale, Giuseppe
AU - Cammarano, Piero
AU - Tiboni, Orsola
PY - 1990/4
Y1 - 1990/4
N2 - The glnA gene of the thermophilic sulphur-dependent archaebacterium Sulfolobus solfataricus was identified by hybridization with the corresponding gene of the cyanobacterium Spirulina platensis and cloned in Escherichia coli. The nucleotide sequence of the 1696 bp DNA fragment containing the structural gene for glutamine synthetase was determined, and the derived amino acid sequence (471 residues) was compared to the sequences of glutamine synthetases from eubacteria and eukaryotes. The homology between the archaebacterial and the eubacterial enzymes is higher (42%-49%) than that found with the eukaryotic counterpart (less than 20%). This was true also when the five most conserved regions, which it is possible to identify in both eubacterial and eukaryotic glutamine synthetases, were analysed.
AB - The glnA gene of the thermophilic sulphur-dependent archaebacterium Sulfolobus solfataricus was identified by hybridization with the corresponding gene of the cyanobacterium Spirulina platensis and cloned in Escherichia coli. The nucleotide sequence of the 1696 bp DNA fragment containing the structural gene for glutamine synthetase was determined, and the derived amino acid sequence (471 residues) was compared to the sequences of glutamine synthetases from eubacteria and eukaryotes. The homology between the archaebacterial and the eubacterial enzymes is higher (42%-49%) than that found with the eukaryotic counterpart (less than 20%). This was true also when the five most conserved regions, which it is possible to identify in both eubacterial and eukaryotic glutamine synthetases, were analysed.
KW - Archaebacteria
KW - Glutamine synthetase
KW - Nuxleotide sequence
KW - Phylogeny
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U2 - 10.1007/BF00261719
DO - 10.1007/BF00261719
M3 - Article
C2 - 1973523
AN - SCOPUS:0025343653
VL - 221
SP - 187
EP - 194
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 2
ER -