Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5

Mika J. Wallén; Mikko O. Laukkanen; Markku S. Kulomaa

doi:10.1016/0378-1119(95)00187-B

Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5

Mika J. Wallén, Mikko O. Laukkanen, Markku S. Kulomaa

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Abstract

The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91-95%) with the previously isolated Avd-related genes 1-5 (Avrl-Avr5). Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.

Original language	English
Pages (from-to)	205-209
Number of pages	5
Journal	Gene
Volume	161
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00187-B
Publication status	Published - Aug 19 1995

Keywords

amino-acid sequence
biotin-binding protein
Gallus gallus
nucleotide sequence
PCR amplification
phylogenetic analysis
Recombinant DNA

ASJC Scopus subject areas

Genetics

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title = "Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5",

abstract = "The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91-95%) with the previously isolated Avd-related genes 1-5 (Avrl-Avr5). Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.",

keywords = "amino-acid sequence, biotin-binding protein, Gallus gallus, nucleotide sequence, PCR amplification, phylogenetic analysis, Recombinant DNA",

author = "Wall{\'e}n, {Mika J.} and Laukkanen, {Mikko O.} and Kulomaa, {Markku S.}",

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T1 - Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5

AU - Wallén, Mika J.

AU - Laukkanen, Mikko O.

AU - Kulomaa, Markku S.

PY - 1995/8/19

Y1 - 1995/8/19

N2 - The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91-95%) with the previously isolated Avd-related genes 1-5 (Avrl-Avr5). Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.

AB - The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91-95%) with the previously isolated Avd-related genes 1-5 (Avrl-Avr5). Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.

KW - amino-acid sequence

KW - biotin-binding protein

KW - Gallus gallus

KW - nucleotide sequence

KW - PCR amplification

KW - phylogenetic analysis

KW - Recombinant DNA

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Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5

Abstract

Keywords

ASJC Scopus subject areas

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