Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5

Mika J. Wallén, Mikko O. Laukkanen, Markku S. Kulomaa

Research output: Contribution to journalArticlepeer-review

Abstract

The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91-95%) with the previously isolated Avd-related genes 1-5 (Avrl-Avr5). Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalGene
Volume161
Issue number2
DOIs
Publication statusPublished - Aug 19 1995

Keywords

  • amino-acid sequence
  • biotin-binding protein
  • Gallus gallus
  • nucleotide sequence
  • PCR amplification
  • phylogenetic analysis
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

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