Abstract
The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91-95%) with the previously isolated Avd-related genes 1-5 (Avrl-Avr5). Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.
Original language | English |
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Pages (from-to) | 205-209 |
Number of pages | 5 |
Journal | Gene |
Volume | 161 |
Issue number | 2 |
DOIs | |
Publication status | Published - Aug 19 1995 |
Keywords
- amino-acid sequence
- biotin-binding protein
- Gallus gallus
- nucleotide sequence
- PCR amplification
- phylogenetic analysis
- Recombinant DNA
ASJC Scopus subject areas
- Genetics