Cloning, chromosome mapping and functional characterization of a human homologue of murine Gtse-1 (B99) gene

M. Monte, L. Collavin, D. Lazarevic, R. Utrera, T. A. Dragani, C. Schneider

Research output: Contribution to journalArticle

Abstract

Murine Gtse-1 (G2 and S phase expressed protein), previously named B99, is a wt-p53 inducible gene that encodes a microtubule-localized protein which is able to induce G2/M phase accumulation when ectopically expressed. Here we report the cloning and characterization of a new cDNA (GTSE-1) encoding a human homologue of the mouse Gtse-1 protein. Chromosome mapping of mouse and human genes assigned Gtse-1 to chromosome 15 and GTSE-1 to chromosome 22q13.2-q13.3 in a region with conserved synteny to that where Gtse-1 mapped. Analysis of the genomic structure revealed that GTSE-1 contains at least 11 exons and 10 introns, spanning ~33kb of genomic DNA. Similar to murine Gtse- 1, the product of GTSE-1 localized to the microtubules, was able to delay G2/M progression when ectopically expressed and was cell cycle regulated. Taken together, these results indicate GTSE-1 as the human functional homologue of murine Gtse-1. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalGene
Volume254
Issue number1-2
DOIs
Publication statusPublished - Aug 22 2000

Keywords

  • Cell cycle
  • G/M arrest
  • Human chr.22q13
  • Microtubules
  • Mouse chr.15

ASJC Scopus subject areas

  • Genetics

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