Cloning, expression and electrophysiological characterization of glycine receptor alpha subunit from zebrafish

B. David-Watine, C. Goblet, D. De Saint Jan, S. Fucile, V. Devignot, P. Bregestovski, H. Korn

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The glycine receptor is a ligand-gated anion channel protein, providing inhibitory drive within the nervous system. We report here the isolation and functional characterization of a novel α subunit (αZ1) of the glycine receptor from adult zebrafish (Danio rerio) brain. The predicted amino acid sequence is 86%, 81% and 77% identical to mammalian isoforms α1, α3 and α2, respectively. αZ1 exhibits many of the molecular features of mammalian α1, but the sequence patterns in the M4 and C-terminal domains are more similar to α2/α3. Phylogenetic analysis indicates that αZ1 is more closely related to the mammalian α1 subunits, being positioned, however, on a distinct branch. The αZ1 messenger RNA is 9.5 kb, similar to that described previously for α1 messenger RNAs. When expressed in Xenopus oocytes or a human cell line (BOSC 23), αZ1 forms a homomeric receptor which is activated by glycine and antagonized by strychnine. This receptor demonstrates unexpectedly high sensitivity to taurine and can also be activated by GABA. These results are consistent with physiological findings in lamprey and goldfish, and they suggest that this teleost fish glycine receptor displays a lower selectivity to neurotransmitters than that reported for glycine mammalian receptors.

Original languageEnglish
Pages (from-to)303-317
Number of pages15
JournalNeuroscience
Volume90
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Glycine Receptors
Zebrafish
Organism Cloning
Ligand-Gated Ion Channels
Lampreys
Strychnine
Messenger RNA
Goldfish
Taurine
Xenopus
Glycine
gamma-Aminobutyric Acid
Nervous System
Oocytes
Anions
Neurotransmitter Agents
Amino Acid Sequence
Protein Isoforms
Fishes
Cell Line

Keywords

  • GABA
  • Glycine receptor
  • Oocyte expression
  • Receptor phylogeny
  • Taurine
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cloning, expression and electrophysiological characterization of glycine receptor alpha subunit from zebrafish. / David-Watine, B.; Goblet, C.; De Saint Jan, D.; Fucile, S.; Devignot, V.; Bregestovski, P.; Korn, H.

In: Neuroscience, Vol. 90, No. 1, 1999, p. 303-317.

Research output: Contribution to journalArticle

David-Watine, B, Goblet, C, De Saint Jan, D, Fucile, S, Devignot, V, Bregestovski, P & Korn, H 1999, 'Cloning, expression and electrophysiological characterization of glycine receptor alpha subunit from zebrafish', Neuroscience, vol. 90, no. 1, pp. 303-317. https://doi.org/10.1016/S0306-4522(98)00430-8
David-Watine, B. ; Goblet, C. ; De Saint Jan, D. ; Fucile, S. ; Devignot, V. ; Bregestovski, P. ; Korn, H. / Cloning, expression and electrophysiological characterization of glycine receptor alpha subunit from zebrafish. In: Neuroscience. 1999 ; Vol. 90, No. 1. pp. 303-317.
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