Cloning, expression and localization of human BM88 shows that it maps to chromosome 11p15.5, a region implicated in Beckwith-Wiedemann syndrome and tumorigenesis

Maria Gaitanou, Pasquale Buanne, Christina Pappa, Niki Georgopoulou, Avgi Mamalaki, Felice Tirone, Rebecca Matsas

Research output: Contribution to journalArticle

Abstract

Porcine BM88 is a neuron-specific protein that enhances neuroblastoma cell differentiation in vitro and may be involved in neuronal differentiation in vivo. Here we report the identification, by Western blotting, of homologous proteins in human and mouse brain and the isolation of their respective cDNAs. Several human and mouse clones were identified in the EST database using porcine BM88 cDNA as a query. A human and a mouse EST clone were chosen for sequencing and were found both to predict a protein of 149 amino acids, with 79.9% reciprocal identity, and 76.4% and 70.7% identities to the porcine protein, respectively. This indicated that the clones corresponded to the human and mouse BM88 homologues. In vitro expression in a cell-free system as well as transient expression in COS7 cells yielded polypeptide products that were recognized by anti-BM88 antibodies and were identical in size to the native BM88 protein. Northern-blot analysis showed a wide distribution of the gene in human brain whereas immunohistochemistry on human brain sections demonstrated that the expression of BM88 is confined to neurons. The initial mapping assignment of human BM88 to chromosome 11p15.5, a region implicated in Beckwith-Wiedemann syndrome and tumorigenesis, was retrieved from the UniGene database maintained at the National Centre for Biotechnology Information (NCBI, Bethesda, MD, U.S.A.). We confirmed this localization by performing fluorescence in situ hybridization on BM88-positive cosmid clones isolated from a human genomic library. These results suggest that BM88 may be a candidate gene for genetic disorders associated with alterations at 11p15.5.

Original languageEnglish
Pages (from-to)715-724
Number of pages10
JournalBiochemical Journal
Volume355
Issue number3
Publication statusPublished - May 1 2001

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Keywords

  • Mouse BM88
  • Neuroblast proliferation
  • Neuron-specific protein
  • Neuronal differentiation
  • Neuronal lineage

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gaitanou, M., Buanne, P., Pappa, C., Georgopoulou, N., Mamalaki, A., Tirone, F., & Matsas, R. (2001). Cloning, expression and localization of human BM88 shows that it maps to chromosome 11p15.5, a region implicated in Beckwith-Wiedemann syndrome and tumorigenesis. Biochemical Journal, 355(3), 715-724.