Cloning immunoglobulin variable domains for expression by the polymerase chain reaction

R. Orlandi, D. H. Gussow, P. T. Jones, G. Winter

Research output: Contribution to journalArticle

Abstract

We have designed a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin heavy and light chain variable domains by the polymerase chain reaction. The primers incorporate restriction sites that allow the cDNA of the variable domains to be force-cloned for sequencing and expression. Here we have applied the technique to clone and sequence the variable domains of five hybridoma antibodies and to express a mouse-human chimeric antibody that binds to the human mammary carcinoma line MCF-7. The technique should also lead to the cloning of antigen-binding specificities directly from immunoglobulin genes.

Original languageEnglish
Pages (from-to)3833-3837
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number10
Publication statusPublished - 1989

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ASJC Scopus subject areas

  • General
  • Genetics

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