Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region

Rossana Pergolizzi, Valentina Appierto, Alessandro Bosetti, GianLuca Debellis, Ermanna Rovida, Ida Biunno

Research output: Contribution to journalArticle

Abstract

To contribute to the isolation of genes within the q24-qter region of the human X chromosome, we screened three cDNA libraries (human fetal brain, liver and skeletal muscle) with a cosmid clone containing a CpG island previously mapped in the q28 region. A full-length 2.1-kb cDNA clone was isolated (XIB); DNA databank searches revealed identity with an EST fragment (XAP-1), residing between the RCP/GCP and G6PD loci. The XIB coding region (909 bp) showed 44% amino acid (aa) identity to pig DNase I. Several conserved residues have been observed between these two genes including aa in the active site. XIB expressed a single transcript in adult heart and skeletal muscle, whereas, in some fetal tissues, two different-sized transcripts were seen. Zoo blot analysis showed a remarkable cross-species conservation. Expression and sequence of this novel gene are reported.

Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalGene
Volume168
Issue number2
DOIs
Publication statusPublished - Feb 12 1996

Keywords

  • Automated DNA sequencing
  • Homology search
  • Human X chromosome

ASJC Scopus subject areas

  • Genetics

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    Pergolizzi, R., Appierto, V., Bosetti, A., Debellis, G., Rovida, E., & Biunno, I. (1996). Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region. Gene, 168(2), 267-270. https://doi.org/10.1016/0378-1119(95)00741-5