Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: New potential mechanisms of drug related toxicity

Maurizio Fanciulli, Tiziana Bruno, Cristina Cerboni, Francesco Bonetto, Carla Iacobini, Luigi Frati, Mario Piccoli, Aristide Floridi, Angela Santoni, Antonello Punturieri

Research output: Contribution to journalArticle

Abstract

Using the differential display PCR method, we have isolated an mRNA downregulated in doxorubicin resistant human cell lines. The full length cDNA clone was identified as the human homologue of yeast RPB11 subunit of RNA polymerase II. Northern blot analysis of normal tissues detected a particularly high expression of RPB11 mRNA in heart and skeletal muscle. Reduction of this mRNA expression was observed in all the cell lines tested after drug treatment and was paralleled by a similar decrease of the protein levels. These findings suggest that doxorubicin may exert in vivo specific inhibitory effects on a major component of the transcription machinery.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalFEBS Letters
Volume384
Issue number1
DOIs
Publication statusPublished - Apr 8 1996

Keywords

  • Differential display PCR
  • Doxorubicin
  • Drug toxicity
  • RNA polymerase II

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Fanciulli, M., Bruno, T., Cerboni, C., Bonetto, F., Iacobini, C., Frati, L., Piccoli, M., Floridi, A., Santoni, A., & Punturieri, A. (1996). Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: New potential mechanisms of drug related toxicity. FEBS Letters, 384(1), 48-52. https://doi.org/10.1016/0014-5793(96)00277-3