Cloning of GPR37, a gene located on chromosome 7 encoding a putative g- protein-coupled peptide receptor, from a human frontal brain EST library

Daniela Marazziti, Elisabetta Golini, Angela Gallo, Maria Stella Lombardi, Rafaele Matteoni, Glauco P. Tocchini-Valentini

Research output: Contribution to journalArticle

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Abstract

A cDNA sequence encoding a putative peptide-specific G-protein-coupled receptor (GPR37) was isolated from a set of human brain frontal lobe expressed sequence tags. The GPR37 cDNA predicts a single open reading frame coding for a 613-amino-acid protein with seven hydrophobic transmembrane domains. The GPR37 genomic sequence was mapped to chromosome 7q31, and it was isolated upon screening of a chromosome 7-specific genomic library. The GPR37 gene spans more than 25 kb and contains two exons and a single intron which interrupts the GPR37 cDNA within the sequence encoding the presumed third transmembrane domain. Northern blot analysis with GPR37 probes revealed a main 3.8-kb mRNA and a less abundant 8-kb mRNA, both expressed in human brain tissues, particularly in corpus callosum, medulla, putamen, and caudate nucleus. The lowest level of expression was detected in cerebellum. The 3.8- kb mRNA is also less abundantly expressed in liver and placenta. Although the ligand for the putative GPR37 receptor has not been identified, its deduced amino acid sequence shows a high degree of homology (~40% in the transmembrane regions) with most mammalian peptide-specific G-protein- coupled receptors and particularly with the human endothelin-B bombesin-BB1, and bombesin-BB2 receptors.

Original languageEnglish
Pages (from-to)68-77
Number of pages10
JournalGenomics
Volume45
Issue number1
DOIs
Publication statusPublished - Oct 1 1997

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Chromosomes, Human, Pair 7
Peptide Receptors
Expressed Sequence Tags
Organism Cloning
Complementary DNA
G-Protein-Coupled Receptors
Messenger RNA
Brain
Bombesin Receptors
Genes
Bombesin
Peptides
Proteins
Genomic Library
Corpus Callosum
Caudate Nucleus
Putamen
Endothelins
Frontal Lobe
Northern Blotting

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning of GPR37, a gene located on chromosome 7 encoding a putative g- protein-coupled peptide receptor, from a human frontal brain EST library. / Marazziti, Daniela; Golini, Elisabetta; Gallo, Angela; Lombardi, Maria Stella; Matteoni, Rafaele; Tocchini-Valentini, Glauco P.

In: Genomics, Vol. 45, No. 1, 01.10.1997, p. 68-77.

Research output: Contribution to journalArticle

Marazziti, Daniela ; Golini, Elisabetta ; Gallo, Angela ; Lombardi, Maria Stella ; Matteoni, Rafaele ; Tocchini-Valentini, Glauco P. / Cloning of GPR37, a gene located on chromosome 7 encoding a putative g- protein-coupled peptide receptor, from a human frontal brain EST library. In: Genomics. 1997 ; Vol. 45, No. 1. pp. 68-77.
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