Abstract
Pentraxins, which include C reactive protein (CRP) and serum amyloid P component (SAP), are prototypic acute phase reactants that serve as indicators of inflammatory reactions. Here we report genomic and cDNA cloning of mouse ptx3 (mptx3), a member of the pentraxin gene family and characterize its extrahepatic expression in vitro and in vivo. mptx3 is organized into three exons on chromosome 3: the first (43 aa) and second exon (175 aa) code for the signal peptide and for a protein portion with no high similarity to known sequences, the third (203 aa) for a domain related to classical pentraxins, which contains the 'pentraxin family signature.' Analysis of the N terminal portion predicts a predominantly α helical structure, while the pentraxin domain of ptx3 is accommodated comfortably in the tertiary structure fold of SAP. Normal and transformed fibroblasts, undifferentiated and differentiated myoblasts, normal endothelial cells, and mononuclear phagocytes express mptx3 mRNA and release the protein in vitro on exposure to interleukin-1β (IL-1β) and tumor necrosis factor (TNF)α. mptx3 was induced by bacterial lipopolysaccharide in vivo in a variety of organs and, most strongly, in the vascular endothelium of skeletal muscle and heart. Thus, mptx3 shows a distinct pattern of in vivo expression indicative of a significant role in cardiovascular and inflammatory pathology.
| Original language | English |
|---|---|
| Pages (from-to) | 1862-1872 |
| Number of pages | 11 |
| Journal | Blood |
| Volume | 87 |
| Issue number | 5 |
| Publication status | Published - Mar 1 1996 |
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ASJC Scopus subject areas
- Hematology
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Cloning of mouse ptx3, a new member of the pentraxin gene family expressed at extrahepatic sites. / Introna, Martino; Vidal Alles, Victor; Castellano, Marina; Picardi, Giuseppina; De Gioia, Luca; Bottazzi, Barbara; Peri, Giuseppe; Breviario, Ferruccio; Salmona, Mario; De Gregorio, Laura; Dragani, Tommaso A.; Srinivasan, Narayanaswamy; Blundell, Tom L.; Hamilton, Thomas A.; Mantovani, Alberto.
In: Blood, Vol. 87, No. 5, 01.03.1996, p. 1862-1872.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning of mouse ptx3, a new member of the pentraxin gene family expressed at extrahepatic sites
AU - Introna, Martino
AU - Vidal Alles, Victor
AU - Castellano, Marina
AU - Picardi, Giuseppina
AU - De Gioia, Luca
AU - Bottazzi, Barbara
AU - Peri, Giuseppe
AU - Breviario, Ferruccio
AU - Salmona, Mario
AU - De Gregorio, Laura
AU - Dragani, Tommaso A.
AU - Srinivasan, Narayanaswamy
AU - Blundell, Tom L.
AU - Hamilton, Thomas A.
AU - Mantovani, Alberto
PY - 1996/3/1
Y1 - 1996/3/1
N2 - Pentraxins, which include C reactive protein (CRP) and serum amyloid P component (SAP), are prototypic acute phase reactants that serve as indicators of inflammatory reactions. Here we report genomic and cDNA cloning of mouse ptx3 (mptx3), a member of the pentraxin gene family and characterize its extrahepatic expression in vitro and in vivo. mptx3 is organized into three exons on chromosome 3: the first (43 aa) and second exon (175 aa) code for the signal peptide and for a protein portion with no high similarity to known sequences, the third (203 aa) for a domain related to classical pentraxins, which contains the 'pentraxin family signature.' Analysis of the N terminal portion predicts a predominantly α helical structure, while the pentraxin domain of ptx3 is accommodated comfortably in the tertiary structure fold of SAP. Normal and transformed fibroblasts, undifferentiated and differentiated myoblasts, normal endothelial cells, and mononuclear phagocytes express mptx3 mRNA and release the protein in vitro on exposure to interleukin-1β (IL-1β) and tumor necrosis factor (TNF)α. mptx3 was induced by bacterial lipopolysaccharide in vivo in a variety of organs and, most strongly, in the vascular endothelium of skeletal muscle and heart. Thus, mptx3 shows a distinct pattern of in vivo expression indicative of a significant role in cardiovascular and inflammatory pathology.
AB - Pentraxins, which include C reactive protein (CRP) and serum amyloid P component (SAP), are prototypic acute phase reactants that serve as indicators of inflammatory reactions. Here we report genomic and cDNA cloning of mouse ptx3 (mptx3), a member of the pentraxin gene family and characterize its extrahepatic expression in vitro and in vivo. mptx3 is organized into three exons on chromosome 3: the first (43 aa) and second exon (175 aa) code for the signal peptide and for a protein portion with no high similarity to known sequences, the third (203 aa) for a domain related to classical pentraxins, which contains the 'pentraxin family signature.' Analysis of the N terminal portion predicts a predominantly α helical structure, while the pentraxin domain of ptx3 is accommodated comfortably in the tertiary structure fold of SAP. Normal and transformed fibroblasts, undifferentiated and differentiated myoblasts, normal endothelial cells, and mononuclear phagocytes express mptx3 mRNA and release the protein in vitro on exposure to interleukin-1β (IL-1β) and tumor necrosis factor (TNF)α. mptx3 was induced by bacterial lipopolysaccharide in vivo in a variety of organs and, most strongly, in the vascular endothelium of skeletal muscle and heart. Thus, mptx3 shows a distinct pattern of in vivo expression indicative of a significant role in cardiovascular and inflammatory pathology.
UR - http://www.scopus.com/inward/record.url?scp=13344261951&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13344261951&partnerID=8YFLogxK
M3 - Article
C2 - 8634434
AN - SCOPUS:13344261951
VL - 87
SP - 1862
EP - 1872
JO - Blood
JF - Blood
SN - 0006-4971
IS - 5
ER -