Cloning of PC3B, a novel member of the PC3/BTG/TOB family of growth inhibitory genes, highly expressed in the olfactory epithelium

Pasquale Buanne, Giuseppina Corrente, Laura Micheli, Antonella Palena, Patrizia Lavia, Corrado Spadafora, Madepalli Krishnappa Lakshmana, Alessandra Rinaldi, Sandro Banfi, Michala Quarto, Alessandro Bulfone, Felice Tirone

Research output: Contribution to journalArticle

Abstract

We identified in the EST database routine and human sequences similar, but not identical, to the members of the PC3/BTG/TOB family of cell cycle inhibitors. A conserved domain (aa 50-68) of the PC3 protein, the prototype member of the family, was used as a query. That domain has been shown by us to be necessary for the antiproliferative activity of PC3. A routine EST clone and a highly homologous human EST clone, containing the entire ORF, were chosen for sequencing. Comparison to databases and a phylogenetic tree analysis indicated thai these EST clones are the mouse and human homologues of a gene that represents a novel member of the PC3/BTG/TOB family. This gene, named PC3B, is endowed with marked antiproliferative activity, being able to induce G1 arrest, and is highly expressed in testis, in oocyte, and in preimplantation embryos. Analysis of its expression during routine development indicated a specific localization in the olfactory epithelium at midgestation, suggesting that PC3B might be involved in the differentiation of this neuronal structure. Human PC3B mapped to chromosome 11q23, as indicated by radiation hybrid analysis. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalGenomics
Volume68
Issue number3
DOIs
Publication statusPublished - Sep 15 2000

ASJC Scopus subject areas

  • Genetics

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    Buanne, P., Corrente, G., Micheli, L., Palena, A., Lavia, P., Spadafora, C., Lakshmana, M. K., Rinaldi, A., Banfi, S., Quarto, M., Bulfone, A., & Tirone, F. (2000). Cloning of PC3B, a novel member of the PC3/BTG/TOB family of growth inhibitory genes, highly expressed in the olfactory epithelium. Genomics, 68(3), 253-263. https://doi.org/10.1006/geno.2000.6288