Cloning of taxadiene synthase gene into Arabidopsis thaliana (ecotype Columbia-0)

Sajjad Khani, Mohammad Mehdi Sohani, Nasser Mahna, Jaleh Barar, Mohammad Saeed Hejazi, Hossein Nazemieh, Sina Atashpaz, Mohammad Reza Dadpour, Yadollah Omidi

Research output: Contribution to journalArticlepeer-review

Abstract

Paclitaxel (Taxol), a complex diterpenoid, produced by yew tree (Taxus sp.) is the most important chemotherapeutic agent that is widely used against a variety of malignancies such as ovarian and breast cancers. However, destructive methods for its production from natural resources together with currently used low-yielding industrial production systems via total synthesis or semi-synthesis have led researchers to invent a robust alternative biological production system using biotechnological approaches. The first committed step in taxol biosynthesis pathway is the production of taxadiene from geranylgeranyl diphosphate (GGPP) catalyzed by the plastid-localized enzyme taxadiene synthase (TXS). In this research, an attempt was made to evaluate the effects of the first critical enzyme in the taxol biosynthesis pathway on Arabidopsis plant through the expression of taxadiene synthase gene under the control of a dexamethasone-inducible promoter. To achieve this goal, Arabidopsis plants (ecotype Columbia-0) were transformed with the construct pTA-TXS-His via floral dip method using Agrobacterium tumefaciens AGL1. The transformed plants were confirmed using the PCR reaction amplifying an 800 bp fragment of the cloned gene. Upon these findings, a proposal was made that biotechnological strategies could be utilized for the production of taxol components.

Original languageEnglish
Pages (from-to)1734-1740
Number of pages7
JournalAfrican Journal of Biotechnology
Volume9
Issue number12
Publication statusPublished - Mar 22 2010

Keywords

  • Arabidopsis
  • Geranylgeranyl diphosphate
  • Paclitaxel
  • Taxadiene synthase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science

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