Cloning of the cDNAs coding for two novel molybdo-flavoproteins showing high similarity with aldehyde oxidase and xanthine oxidoreductase

M. Terao, M. Kurosaki, G. Saltini, S. Demontis, M. Marini, M. Salmona, E. Garattini

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Abstract

The cDNAs coding for two novel mouse molybdo-flavoproteins, AOH1 and AOH2 (aldehyde oxidase homolog 1 and 2), were isolated. The AOH1 and AOH2 cDNAs code for polypeptides of 1336 amino acids. The two proteins have similar primary structure and show striking amino acid identity with aldehyde oxidase and xanthine oxidoreductase, two other molybdo-flavoenzymes. AOH1 and AOH2 contain consensus sequences for a molybdopterin-binding site and two distinct 2Fe-2S redox centers. In its native conformation, AOH1 has a molecular weight consistent with a homotetrameric structure. Transfection of the AOH1 and AOH2 cDNAs results in the production of proteins with phenanthridine but not hypoxanthine oxidizing activity. Furthermore, the AOH1 protein has benzaldehyde oxidizing activity with electrophoretic characteristics identical to those of a previously identified aldehyde oxidase isoenzyme (Holmes, R. S. (1979) Biochem. Genet. 17, 517-528). The AOH1 transcript is expressed in the hepatocytes of the adult and fetal liver and in spermatogonia. In liver, the AOH1 protein is synthesized in a gender-specific fashion. The expression of AOH2 is limited to keratinized epithelia and the basal layer of the epidermis and hair folliculi. The selective cell and tissue distribution of AOH1 and AOH2 mRNAs is consistent with the localization of the respective protein products.

Original languageEnglish
Pages (from-to)30690-30700
Number of pages11
JournalJournal of Biological Chemistry
Volume275
Issue number39
Publication statusPublished - Sep 29 2000

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Aldehyde Oxidoreductases
Aldehyde Oxidase
Xanthine Dehydrogenase
Flavoproteins
Cloning
Organism Cloning
Complementary DNA
Proteins
Liver
Phenanthridines
Viverridae
Amino Acids
Spermatogonia
Hypoxanthine
Consensus Sequence
Tissue Distribution
Epidermis
Hair
Isoenzymes
Oxidation-Reduction

ASJC Scopus subject areas

  • Biochemistry

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Cloning of the cDNAs coding for two novel molybdo-flavoproteins showing high similarity with aldehyde oxidase and xanthine oxidoreductase. / Terao, M.; Kurosaki, M.; Saltini, G.; Demontis, S.; Marini, M.; Salmona, M.; Garattini, E.

In: Journal of Biological Chemistry, Vol. 275, No. 39, 29.09.2000, p. 30690-30700.

Research output: Contribution to journalArticle

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AU - Garattini, E.

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