Cloning of the gene encoding the δ subunit of the human T-cell receptor reveals its physical organization within the α-subunit locus and its involvement in chromosome translocations in T-cell malignancy

M. Isobe, G. Russo, F. G. Haluska, C. M. Croce

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

By taking advantage of 'chromosomal walking' techniques, we have obtained clones that encompass the T-cell receptor (TCR) δ-chain gene. We analyzed clones spanning the entire J(α) region extending 115 kilobases 5' of the TCR α-chain constant region and have shown that the TCR δ-chain gene is located over 80 kilobases 5' of C(α). TCR δ-chain gene is rearranged in the γ/δ-expressing T-cell line Peer and is delected in α/β-expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C(δ) and J(δ) segments. Furthermore, we have analyzed a t(8;14)-(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J(δ) segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly cloned TCR δ chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.

Original languageEnglish
Pages (from-to)3933-3937
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number11
Publication statusPublished - 1988

Fingerprint

T-Cell Receptor Genes
T-Cell Antigen Receptor
Organism Cloning
Clone Cells
Chromosomes
T-Lymphocytes
Genes
T-Cell Leukemia
Cell Line
Neoplasms
Chromosome Aberrations
Walking
Sequence Analysis
Complementary DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{64cb61c8cc9144299b0f434ea1dced13,
title = "Cloning of the gene encoding the δ subunit of the human T-cell receptor reveals its physical organization within the α-subunit locus and its involvement in chromosome translocations in T-cell malignancy",
abstract = "By taking advantage of 'chromosomal walking' techniques, we have obtained clones that encompass the T-cell receptor (TCR) δ-chain gene. We analyzed clones spanning the entire J(α) region extending 115 kilobases 5' of the TCR α-chain constant region and have shown that the TCR δ-chain gene is located over 80 kilobases 5' of C(α). TCR δ-chain gene is rearranged in the γ/δ-expressing T-cell line Peer and is delected in α/β-expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C(δ) and J(δ) segments. Furthermore, we have analyzed a t(8;14)-(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J(δ) segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly cloned TCR δ chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.",
author = "M. Isobe and G. Russo and Haluska, {F. G.} and Croce, {C. M.}",
year = "1988",
language = "English",
volume = "85",
pages = "3933--3937",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "11",

}

TY - JOUR

T1 - Cloning of the gene encoding the δ subunit of the human T-cell receptor reveals its physical organization within the α-subunit locus and its involvement in chromosome translocations in T-cell malignancy

AU - Isobe, M.

AU - Russo, G.

AU - Haluska, F. G.

AU - Croce, C. M.

PY - 1988

Y1 - 1988

N2 - By taking advantage of 'chromosomal walking' techniques, we have obtained clones that encompass the T-cell receptor (TCR) δ-chain gene. We analyzed clones spanning the entire J(α) region extending 115 kilobases 5' of the TCR α-chain constant region and have shown that the TCR δ-chain gene is located over 80 kilobases 5' of C(α). TCR δ-chain gene is rearranged in the γ/δ-expressing T-cell line Peer and is delected in α/β-expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C(δ) and J(δ) segments. Furthermore, we have analyzed a t(8;14)-(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J(δ) segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly cloned TCR δ chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.

AB - By taking advantage of 'chromosomal walking' techniques, we have obtained clones that encompass the T-cell receptor (TCR) δ-chain gene. We analyzed clones spanning the entire J(α) region extending 115 kilobases 5' of the TCR α-chain constant region and have shown that the TCR δ-chain gene is located over 80 kilobases 5' of C(α). TCR δ-chain gene is rearranged in the γ/δ-expressing T-cell line Peer and is delected in α/β-expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C(δ) and J(δ) segments. Furthermore, we have analyzed a t(8;14)-(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J(δ) segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly cloned TCR δ chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.

UR - http://www.scopus.com/inward/record.url?scp=0023942487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023942487&partnerID=8YFLogxK

M3 - Article

VL - 85

SP - 3933

EP - 3937

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11

ER -