Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium

P. A. Bernabei, V. Santini, O. Dal Pozzo, R. Bezzini, V. Gattei, R. Saccardi, G. Rombolà, P. Rossi Ferrini

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.

Original languageEnglish
Pages (from-to)903-906
Number of pages4
JournalExperientia
Volume44
Issue number10
DOIs
Publication statusPublished - Oct 1988

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Serum-Free Culture Media
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Growth
Serum
Transferrin
Conditioned Culture Medium
Hydrocortisone
Albumins
Intercellular Signaling Peptides and Proteins
Stem Cells
Clone Cells
Insulin
Lipids

Keywords

  • Acute non-lymphocytic leukemia
  • serum-free medium
  • tumor cell cloning

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bernabei, P. A., Santini, V., Dal Pozzo, O., Bezzini, R., Gattei, V., Saccardi, R., ... Ferrini, P. R. (1988). Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium. Experientia, 44(10), 903-906. https://doi.org/10.1007/BF01941197

Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium. / Bernabei, P. A.; Santini, V.; Dal Pozzo, O.; Bezzini, R.; Gattei, V.; Saccardi, R.; Rombolà, G.; Ferrini, P. Rossi.

In: Experientia, Vol. 44, No. 10, 10.1988, p. 903-906.

Research output: Contribution to journalArticle

Bernabei, PA, Santini, V, Dal Pozzo, O, Bezzini, R, Gattei, V, Saccardi, R, Rombolà, G & Ferrini, PR 1988, 'Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium', Experientia, vol. 44, no. 10, pp. 903-906. https://doi.org/10.1007/BF01941197
Bernabei PA, Santini V, Dal Pozzo O, Bezzini R, Gattei V, Saccardi R et al. Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium. Experientia. 1988 Oct;44(10):903-906. https://doi.org/10.1007/BF01941197
Bernabei, P. A. ; Santini, V. ; Dal Pozzo, O. ; Bezzini, R. ; Gattei, V. ; Saccardi, R. ; Rombolà, G. ; Ferrini, P. Rossi. / Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium. In: Experientia. 1988 ; Vol. 44, No. 10. pp. 903-906.
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