Abstract
We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.
| Original language | English |
|---|---|
| Pages (from-to) | 903-906 |
| Number of pages | 4 |
| Journal | Experientia |
| Volume | 44 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1988 |
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Keywords
- Acute non-lymphocytic leukemia
- serum-free medium
- tumor cell cloning
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium. / Bernabei, P. A.; Santini, V.; Dal Pozzo, O.; Bezzini, R.; Gattei, V.; Saccardi, R.; Rombolà, G.; Ferrini, P. Rossi.
In: Experientia, Vol. 44, No. 10, 10.1988, p. 903-906.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium
AU - Bernabei, P. A.
AU - Santini, V.
AU - Dal Pozzo, O.
AU - Bezzini, R.
AU - Gattei, V.
AU - Saccardi, R.
AU - Rombolà, G.
AU - Ferrini, P. Rossi
PY - 1988/10
Y1 - 1988/10
N2 - We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.
AB - We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.
KW - Acute non-lymphocytic leukemia
KW - serum-free medium
KW - tumor cell cloning
UR - http://www.scopus.com/inward/record.url?scp=0023732931&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023732931&partnerID=8YFLogxK
U2 - 10.1007/BF01941197
DO - 10.1007/BF01941197
M3 - Article
C2 - 3181378
AN - SCOPUS:0023732931
VL - 44
SP - 903
EP - 906
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
SN - 1420-682X
IS - 10
ER -