Abstract
We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.
Original language | English |
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Pages (from-to) | 903-906 |
Number of pages | 4 |
Journal | Experientia |
Volume | 44 |
Issue number | 10 |
DOIs | |
Publication status | Published - Oct 1988 |
Keywords
- Acute non-lymphocytic leukemia
- serum-free medium
- tumor cell cloning
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)