Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium

P. A. Bernabei, V. Santini, O. Dal Pozzo, R. Bezzini, V. Gattei, R. Saccardi, G. Rombolà, P. Rossi Ferrini

Research output: Contribution to journalArticlepeer-review

Abstract

We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.

Original languageEnglish
Pages (from-to)903-906
Number of pages4
JournalExperientia
Volume44
Issue number10
DOIs
Publication statusPublished - Oct 1988

Keywords

  • Acute non-lymphocytic leukemia
  • serum-free medium
  • tumor cell cloning

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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