Abstract
Several studies have demonstrated the existence of a murine NK1.1+ αβ T cell subset expressing Vα14+ TCR α-chains with highly conserved invariant junctional sequences and able to secrete Th2 cytokines when exposed to CD1+ stimulator cells. In humans, α/β T cells carrying invariant Vα24+ TCR α-chains highly homologous to those expressed by murine NK1.1 cells have been recently described. Here we show that these cells (referred to as Vα24inv T cells) and murine NK1.1+ αβ T cells resemble each other in several ways. First, like their murine counterparts, T cells expressing high levels of Vα24inv TCRs can be either CD4-CD8- double negative (DN) or CD4+, but they never express heterodimeric CD8 molecules. Second, most Vα24inv T cells are brightly stained by NKRP1-specific mAb but not by mAb directed against other type II transmembrane proteins of the NK complex. Third, DN and particularly CD4+ Vα24inv T cells are greatly enriched for IL-4 producers. The concomitant expression of highly conserved TCRs of a particular set of NK markers and of Th2 cytokines in human and murine αβ T cells suggests a coordinate acquisition of these phenotypic and functional properties. Furthermore, the relatively high frequency of human Vα24inv T cells, which are presently shown to represent on average 1/500 PBL, and the high interindividual variations of the size of this cell subset under physiologic conditions go for a major role played by αβ T cells carrying invariant TCR in a large array of immune responses.
| Original language | English |
|---|---|
| Pages (from-to) | 5603-5611 |
| Number of pages | 9 |
| Journal | Journal of Immunology |
| Volume | 158 |
| Issue number | 12 |
| Publication status | Published - Jun 15 1997 |
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ASJC Scopus subject areas
- Immunology
Cite this
Close Phenotypic and Functional Similarities Between Human and Murine αβ T Cells Expressing Invariant TCR α-Chains. / Davodeau, François; Peyrat, Marie Alix; Necker, Antje; Dominici, Roberto; Blanchard, Frédéric; Leget, Corinne; Gaschet, Joelle; Costa, Paola; Jacques, Yannick; Godard, Anne; Vié, Henri; Poggi, Alessandro; Romagné, François; Bonneville, Marc.
In: Journal of Immunology, Vol. 158, No. 12, 15.06.1997, p. 5603-5611.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Close Phenotypic and Functional Similarities Between Human and Murine αβ T Cells Expressing Invariant TCR α-Chains
AU - Davodeau, François
AU - Peyrat, Marie Alix
AU - Necker, Antje
AU - Dominici, Roberto
AU - Blanchard, Frédéric
AU - Leget, Corinne
AU - Gaschet, Joelle
AU - Costa, Paola
AU - Jacques, Yannick
AU - Godard, Anne
AU - Vié, Henri
AU - Poggi, Alessandro
AU - Romagné, François
AU - Bonneville, Marc
PY - 1997/6/15
Y1 - 1997/6/15
N2 - Several studies have demonstrated the existence of a murine NK1.1+ αβ T cell subset expressing Vα14+ TCR α-chains with highly conserved invariant junctional sequences and able to secrete Th2 cytokines when exposed to CD1+ stimulator cells. In humans, α/β T cells carrying invariant Vα24+ TCR α-chains highly homologous to those expressed by murine NK1.1 cells have been recently described. Here we show that these cells (referred to as Vα24inv T cells) and murine NK1.1+ αβ T cells resemble each other in several ways. First, like their murine counterparts, T cells expressing high levels of Vα24inv TCRs can be either CD4-CD8- double negative (DN) or CD4+, but they never express heterodimeric CD8 molecules. Second, most Vα24inv T cells are brightly stained by NKRP1-specific mAb but not by mAb directed against other type II transmembrane proteins of the NK complex. Third, DN and particularly CD4+ Vα24inv T cells are greatly enriched for IL-4 producers. The concomitant expression of highly conserved TCRs of a particular set of NK markers and of Th2 cytokines in human and murine αβ T cells suggests a coordinate acquisition of these phenotypic and functional properties. Furthermore, the relatively high frequency of human Vα24inv T cells, which are presently shown to represent on average 1/500 PBL, and the high interindividual variations of the size of this cell subset under physiologic conditions go for a major role played by αβ T cells carrying invariant TCR in a large array of immune responses.
AB - Several studies have demonstrated the existence of a murine NK1.1+ αβ T cell subset expressing Vα14+ TCR α-chains with highly conserved invariant junctional sequences and able to secrete Th2 cytokines when exposed to CD1+ stimulator cells. In humans, α/β T cells carrying invariant Vα24+ TCR α-chains highly homologous to those expressed by murine NK1.1 cells have been recently described. Here we show that these cells (referred to as Vα24inv T cells) and murine NK1.1+ αβ T cells resemble each other in several ways. First, like their murine counterparts, T cells expressing high levels of Vα24inv TCRs can be either CD4-CD8- double negative (DN) or CD4+, but they never express heterodimeric CD8 molecules. Second, most Vα24inv T cells are brightly stained by NKRP1-specific mAb but not by mAb directed against other type II transmembrane proteins of the NK complex. Third, DN and particularly CD4+ Vα24inv T cells are greatly enriched for IL-4 producers. The concomitant expression of highly conserved TCRs of a particular set of NK markers and of Th2 cytokines in human and murine αβ T cells suggests a coordinate acquisition of these phenotypic and functional properties. Furthermore, the relatively high frequency of human Vα24inv T cells, which are presently shown to represent on average 1/500 PBL, and the high interindividual variations of the size of this cell subset under physiologic conditions go for a major role played by αβ T cells carrying invariant TCR in a large array of immune responses.
UR - http://www.scopus.com/inward/record.url?scp=0031570534&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031570534&partnerID=8YFLogxK
M3 - Article
C2 - 9190907
AN - SCOPUS:0031570534
VL - 158
SP - 5603
EP - 5611
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 12
ER -