Clotting factors and platelets: Immunofluorescence evidence that fibrinogen and factor viii are present in human washed platelets, whereas prothrombin complex factors and factor xiii are lacking

C. Bhtterle, F. Fabris, L. De Marco, G. F. Del Prete, A. Girolami

Research output: Contribution to journalArticlepeer-review

Abstract

Factor I (fibrinogen) and factor VIII were shown, by an indirect immuno-fluorescence technique, to be present in human washed platelets. In the case of fibrinogen, the immunofluorescent pattern had a ‘clod distribution’ up to a 1:128 dilution of the anti-serum. Factor VIII showed a ‘speckled’ pattern up to the 1:64 dilution. Cross-absorption studies confirmed the presence of such factors. Factor II, VII, IX, X and XIII, however, were not found in washed platelets. A diffuse fluorescence was noted using normal rabbit sera, but this was due to a cross-reaction, since it disappeared using a 1:32 diluted sera.

Original languageEnglish
Pages (from-to)171-179
Number of pages9
JournalPathophysiology of Haemostasis and Thrombosis
Volume6
Issue number3
DOIs
Publication statusPublished - 1977

Keywords

  • Clotting factors
  • Platelets-Immunofluorescence

ASJC Scopus subject areas

  • Hematology
  • Physiology (medical)

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