Abstract
To test the hypothesis that clozapine-induced reduction of glutamate transporter-1 (GLT-1) expression is mediated by astrocytes, we studied the effects of clozapine on Glu transporters and Glu uptake in primary astrocyte cultures of the cerebral cortex. Astrocyte cultures treated for 48 h with clozapine exhibited a reduction in GLT-1 levels of about 50%, whereas glutamate-aspartate transporter (GLAST) levels remained unchanged. Glu uptake was also lowered, and this reduction was dose-dependent. Our findings indicate that clozapine reduces GLT-1 expression and function by a mechanism that directly involves astrocytes. A better understanding of the molecular events by which antipsychotics regulate Glu uptake can contribute to identify new targets for the treatment of schizophrenia.
| Original language | English |
|---|---|
| Pages (from-to) | 276-279 |
| Number of pages | 4 |
| Journal | GLIA |
| Volume | 50 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 2005 |
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Keywords
- Antipsychotics
- Astrocytes
- Glutamate transport
- Schizophrenia
ASJC Scopus subject areas
- Immunology
Cite this
Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures. / Vallejo-Illarramendi, Ainara; Torres-Ramos, Mónica; Melone, Marcello; Conti, Fiorenzo; Matute, Carlos.
In: GLIA, Vol. 50, No. 3, 05.2005, p. 276-279.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures
AU - Vallejo-Illarramendi, Ainara
AU - Torres-Ramos, Mónica
AU - Melone, Marcello
AU - Conti, Fiorenzo
AU - Matute, Carlos
PY - 2005/5
Y1 - 2005/5
N2 - To test the hypothesis that clozapine-induced reduction of glutamate transporter-1 (GLT-1) expression is mediated by astrocytes, we studied the effects of clozapine on Glu transporters and Glu uptake in primary astrocyte cultures of the cerebral cortex. Astrocyte cultures treated for 48 h with clozapine exhibited a reduction in GLT-1 levels of about 50%, whereas glutamate-aspartate transporter (GLAST) levels remained unchanged. Glu uptake was also lowered, and this reduction was dose-dependent. Our findings indicate that clozapine reduces GLT-1 expression and function by a mechanism that directly involves astrocytes. A better understanding of the molecular events by which antipsychotics regulate Glu uptake can contribute to identify new targets for the treatment of schizophrenia.
AB - To test the hypothesis that clozapine-induced reduction of glutamate transporter-1 (GLT-1) expression is mediated by astrocytes, we studied the effects of clozapine on Glu transporters and Glu uptake in primary astrocyte cultures of the cerebral cortex. Astrocyte cultures treated for 48 h with clozapine exhibited a reduction in GLT-1 levels of about 50%, whereas glutamate-aspartate transporter (GLAST) levels remained unchanged. Glu uptake was also lowered, and this reduction was dose-dependent. Our findings indicate that clozapine reduces GLT-1 expression and function by a mechanism that directly involves astrocytes. A better understanding of the molecular events by which antipsychotics regulate Glu uptake can contribute to identify new targets for the treatment of schizophrenia.
KW - Antipsychotics
KW - Astrocytes
KW - Glutamate transport
KW - Schizophrenia
UR - http://www.scopus.com/inward/record.url?scp=20044382110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20044382110&partnerID=8YFLogxK
U2 - 10.1002/glia.20172
DO - 10.1002/glia.20172
M3 - Article
C2 - 15739191
AN - SCOPUS:20044382110
VL - 50
SP - 276
EP - 279
JO - GLIA
JF - GLIA
SN - 0894-1491
IS - 3
ER -