Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures

Ainara Vallejo-Illarramendi, Mónica Torres-Ramos, Marcello Melone, Fiorenzo Conti, Carlos Matute

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

To test the hypothesis that clozapine-induced reduction of glutamate transporter-1 (GLT-1) expression is mediated by astrocytes, we studied the effects of clozapine on Glu transporters and Glu uptake in primary astrocyte cultures of the cerebral cortex. Astrocyte cultures treated for 48 h with clozapine exhibited a reduction in GLT-1 levels of about 50%, whereas glutamate-aspartate transporter (GLAST) levels remained unchanged. Glu uptake was also lowered, and this reduction was dose-dependent. Our findings indicate that clozapine reduces GLT-1 expression and function by a mechanism that directly involves astrocytes. A better understanding of the molecular events by which antipsychotics regulate Glu uptake can contribute to identify new targets for the treatment of schizophrenia.

Original languageEnglish
Pages (from-to)276-279
Number of pages4
JournalGLIA
Volume50
Issue number3
DOIs
Publication statusPublished - May 2005

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Amino Acid Transport System X-AG
Clozapine
Astrocytes
Glutamic Acid
Cerebral Cortex
Antipsychotic Agents
Schizophrenia

Keywords

  • Antipsychotics
  • Astrocytes
  • Glutamate transport
  • Schizophrenia

ASJC Scopus subject areas

  • Immunology

Cite this

Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures. / Vallejo-Illarramendi, Ainara; Torres-Ramos, Mónica; Melone, Marcello; Conti, Fiorenzo; Matute, Carlos.

In: GLIA, Vol. 50, No. 3, 05.2005, p. 276-279.

Research output: Contribution to journalArticle

Vallejo-Illarramendi, A, Torres-Ramos, M, Melone, M, Conti, F & Matute, C 2005, 'Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures', GLIA, vol. 50, no. 3, pp. 276-279. https://doi.org/10.1002/glia.20172
Vallejo-Illarramendi, Ainara ; Torres-Ramos, Mónica ; Melone, Marcello ; Conti, Fiorenzo ; Matute, Carlos. / Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures. In: GLIA. 2005 ; Vol. 50, No. 3. pp. 276-279.
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