Cluster assembled nanostructured TiO2 film mediates efficient and safe retroviral gene transduction in primary adult human melanocyte for ex-vivo gene therapy

Roberta Carbone, Ida Marangi, Andrea Zanardi, Luisa Lanfrancone, Saverio Minucci, Pier Giuseppe Pelicci, L. Giorgetti, E. Clerici, G. Bongiorno, P. Piseri, P. Milani

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Primary human melanocytes represent the precursor cells to melanoma and also specific targets of inherited and acquired pigmentation disorders [1-3]. We have developed a new gene transfer technology on primary adult human melanocytes by means of retroviral infection in absence of any toxic polycations currently employed to improve infection efficency (i.e. polybrene) that, as we show experimentally, induce apoptosis and DNA damage. We have cultured primary human melanocytes on a biocompatible nanostructured TiO2 film, obtained by the deposition of a supersonic beam of titania clusters [4-6], coated by retroviral vectors expressing GFP. By means of a "reverse infection" mechanism we achieved 80% of infection in absence of any toxic effect. We expect that such technology will allow efficient and safe genetic manipulation of primary cells for ex-vivo gene therapy.

Original languageEnglish
Title of host publication2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Pages111-114
Number of pages4
Volume2
Publication statusPublished - 2006
Event2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings - Boston, MA, United States
Duration: May 7 2006May 11 2006

Other

Other2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
CountryUnited States
CityBoston, MA
Period5/7/065/11/06

Keywords

  • Adult human melanocytes
  • Ex-vivo gene therapy
  • Nanostructured TiO films
  • Retroviral gene transduction

ASJC Scopus subject areas

  • Engineering(all)

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