Co-expression of CD133+/CD44+ in human colon cancer and liver metastasis

Antonia Bellizzi, Sinto Sebastian, Pasquale Ceglia, Matteo Centonze, Rosa Divella, Elvira Foglia Manzillo, Amalia Azzariti, Nicola Silvestris, Severino Montemurro, Cosimo Caliandro, Raffaele De Luca, Giuseppe Cicero, Sergio Rizzo, Antonio Russo, Michele Quaranta, Giovanni Simone, Angelo Paradiso

Research output: Contribution to journalArticlepeer-review

Abstract

Although relatively good therapeutic results are achieved in non-advanced cancer, the prognosis of the advanced colon cancer still remains poor, dependent on local or distant recurrence of the disease. One of the factors responsible for recurrence is supposed to be cancer stem cells (CSCs) or tumor-initiating cells, which are a population of cancer cells with ability to perpetuate themselves through self-renewal and to generate differentiated cells, thought to be responsible for tumor recurrence. This study globally approach the possible role of tissue-derived stem cells in the initiation of colon cancer and its metastatic process in the liver. Fresh surgical specimens from colon cancer, non-tumor tissue and liver metastasis were obtained directly from the operating room, examined, and immediately processed. CSCs were selected under serum-free conditions and characterized by CD44 and CD133 expression levels. CD133+/CD44+ cell populations were then investigated in paraffin-embedded tissues and circulating tumor cells isolated from peripheral blood of the same group of colon cancer patients. Our data demonstrate that metastatic properties of cell populations from blood and liver metastasis, differently from primitive tumors, seem to be strictly related to the phenotype CD133 positive and CD44 positive.

Original languageEnglish
Pages (from-to)408-415
Number of pages8
JournalJournal of Cellular Physiology
Volume228
Issue number2
DOIs
Publication statusPublished - Feb 2013

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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