Co-localization of N-acetyl-aspartyl-glutamate in central cholinergic, noradrenergic, and serotonergic neurons.

G. Forloni, R. Grzanna, R. D. Blakely, J. T. Coyle

Research output: Contribution to journalArticle

Abstract

An immunohistochemical technique for simultaneously visualizing two different antigens has been used to investigate the presence of the acidic dipeptide, N-acetyl-aspartyl-glutamate (NAAG), in cholinergic, noradrenergic-adrenergic, and serotonergic neurons within CNS. The brain slices were processed sequentially with purified antisera against NAAG and then monoclonal antibody against choline acetyltransferase (ChAT), a marker for cholinergic neurons, or antiserum against dopamine-beta-hydroxylase (DBH), a marker of noradrenergic-adrenergic neurons, or antiserum against serotonin (5HT). Both antigens were revealed by the peroxidase reaction but with different chromogens, which are easily distinguishable. An intense double staining of NAAG-like immunoreactivity (NAAG-LI) and ChAT was observed in the motoneurons of the spinal cord as well as in the several motor components of cranial nerve nuclei including facial, ambiguus, and trigeminal nuclei. A partial colocalization of NAAG-LI and ChAT was evident in the perikarya of the basal forebrain cholinergic system, whereas cholinergic neurons of the medial septum exhibited only sporadic staining for NAAG-LI. A complete coexistence of NAAG-LI and DBH was observed in the locus coeruleus. Most of the other noradrenergic and adrenergic cell groups of the medulla region exhibited substantial co-localization with the exception of the A2 cell group, which was virtually devoid of NAAG-LI. In the dorsal raphe, only a low percentage of serotonergic neurons stained for NAAG-LI. The co-existence of NAAG-LI and serotonin was more evident in the neurons of the median raphe, although the majority of cells failed to show double staining.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)455-460
Number of pages6
JournalSynapse
Volume1
Issue number5
Publication statusPublished - 1987

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Serotonergic Neurons
Adrenergic Neurons
Cholinergic Neurons
glutamate acetyltransferase
Choline O-Acetyltransferase
Immune Sera
Dopamine beta-Hydroxylase
Staining and Labeling
Cholinergic Agents
Serotonin
N-acetyl-1-aspartylglutamic acid
Trigeminal Nuclei
Antigens
Locus Coeruleus
Cranial Nerves
Dipeptides
Motor Neurons
varespladib methyl
Adrenergic Agents
Peroxidase

ASJC Scopus subject areas

  • Physiology

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Co-localization of N-acetyl-aspartyl-glutamate in central cholinergic, noradrenergic, and serotonergic neurons. / Forloni, G.; Grzanna, R.; Blakely, R. D.; Coyle, J. T.

In: Synapse, Vol. 1, No. 5, 1987, p. 455-460.

Research output: Contribution to journalArticle

Forloni, G. ; Grzanna, R. ; Blakely, R. D. ; Coyle, J. T. / Co-localization of N-acetyl-aspartyl-glutamate in central cholinergic, noradrenergic, and serotonergic neurons. In: Synapse. 1987 ; Vol. 1, No. 5. pp. 455-460.
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