Co-optation of Tandem DNA Repeats for the Maintenance of Mesenchymal Identity

Chiara Balestrieri, Gabriele Alfarano, Marta Milan, Valentina Tosi, Elena Prosperini, Paola Nicoli, Andrea Palamidessi, Giorgio Scita, Giuseppe R. Diaferia, Gioacchino Natoli

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tandem repeats (TRs) are generated by DNA replication errors and retain a high level of instability, which in principle would make them unsuitable for integration into gene regulatory networks. However, the appearance of DNA sequence motifs recognized by transcription factors may turn TRs into functional cis-regulatory elements, thus favoring their stabilization in genomes. Here, we show that, in human cells, the transcriptional repressor ZEB1, which promotes the maintenance of mesenchymal features largely by suppressing epithelial genes and microRNAs, occupies TRs harboring dozens of copies of its DNA-binding motif within genomic loci relevant for maintenance of epithelial identity. The deletion of one such TR caused quasi-mesenchymal cancer cells to reacquire epithelial features, partially recapitulating the effects of ZEB1 gene deletion. These data demonstrate that the high density of identical motifs in TRs can make them suitable platforms for recruitment of transcriptional repressors, thus promoting their exaptation into pre-existing cis-regulatory networks. Tandem repeats, iterated and unstable sequences generated by DNA replication errors, can be integrated into ancient gene regulatory networks controlling mesenchymal identity and stabilized in the human genome.

Original languageEnglish
Pages (from-to)1150-1164.e14
JournalCell
Volume173
Issue number5
DOIs
Publication statusPublished - May 17 2018

Fingerprint

Tandem Repeat Sequences
Genes
Maintenance
DNA
Nucleotide Motifs
Gene Regulatory Networks
DNA Replication
Cells
DNA sequences
Gene Deletion
Human Genome
MicroRNAs
Transcription Factors
Stabilization
Genome
Neoplasms

Keywords

  • epithelial-to-mesenchymal transition
  • gene regulatory networks
  • gene repression
  • pancreatic cancer
  • repetitive elements
  • tandem repeats
  • ZEB1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Co-optation of Tandem DNA Repeats for the Maintenance of Mesenchymal Identity. / Balestrieri, Chiara; Alfarano, Gabriele; Milan, Marta; Tosi, Valentina; Prosperini, Elena; Nicoli, Paola; Palamidessi, Andrea; Scita, Giorgio; Diaferia, Giuseppe R.; Natoli, Gioacchino.

In: Cell, Vol. 173, No. 5, 17.05.2018, p. 1150-1164.e14.

Research output: Contribution to journalArticle

Balestrieri, C, Alfarano, G, Milan, M, Tosi, V, Prosperini, E, Nicoli, P, Palamidessi, A, Scita, G, Diaferia, GR & Natoli, G 2018, 'Co-optation of Tandem DNA Repeats for the Maintenance of Mesenchymal Identity', Cell, vol. 173, no. 5, pp. 1150-1164.e14. https://doi.org/10.1016/j.cell.2018.03.081
Balestrieri, Chiara ; Alfarano, Gabriele ; Milan, Marta ; Tosi, Valentina ; Prosperini, Elena ; Nicoli, Paola ; Palamidessi, Andrea ; Scita, Giorgio ; Diaferia, Giuseppe R. ; Natoli, Gioacchino. / Co-optation of Tandem DNA Repeats for the Maintenance of Mesenchymal Identity. In: Cell. 2018 ; Vol. 173, No. 5. pp. 1150-1164.e14.
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