Cobalamin (vitamin B12) positively regulates interleukin-6 levels in rat cerebrospinal fluid

G. Scalabrino, M. M. Corsi, D. Veber, F. R. Buccellato, G. Pravettoni, A. Manfridi, P. Magni

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously demonstrated that the repeated intracerebroventricular (i.c.v.) microinjection of interleukin-6 (IL-6) prevented the myelinolytic lesions of cobalamin-deficient (Cbl-D) central neuropathy [or subacute combined degeneration (SCD)] in totally gastrectomized (TGX) rats. We therefore hypothesized that cobalamin (Cbl) may actually regulate IL-6 levels in rat cerebrospinal fluid (CSF). We measured IL-6 levels in the CSF of rats made Cbl-D by means of total gastrectomy (TG) or chronic feeding with a Cbl-D diet and killed at different times from the beginning of the experiment, and found that IL-6 levels significantly and progressively decreased over time. Chronic 2-month Cbl administration started 1 week after surgery prevented the decrease in IL-6 levels and, when it was started 2 months after surgery, it significantly increased IL-6 levels, but not to presurgical values. We also investigated whether IL-6 decrease might be ultimately due to the Cbl-deficiency-linked decrease in epidermal growth factor (EGF) synthesis. Repeated i.c.v. administrations of EGF to TGX rats did not modify CSF IL-6 levels. These results, together with those of a previous study showing the preventive effect of IL-6 treatment on SCD lesions, demonstrate that: (i) Cbl selectively regulates CSF IL-6 levels; and (ii) decreased IL-6 availability plays a role in the pathogenesis of the experimental SCD, in which no evidence of inflammatory and/or immunological reaction has been observed.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalJournal of Neuroimmunology
Volume127
Issue number1-2
DOIs
Publication statusPublished - 2002

Keywords

  • Cerebrospinal fluid
  • Cobalamin
  • Cobalamin deficiency
  • Interleukin-6
  • Subacute combined degeneration
  • Vitamin B

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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