Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions

M. Taglialatela; L. M T Canzoniero; S. Amoroso; A. Fatatis; G. F. Di Renzo; L. Annunziato

doi:10.1016/0006-8993(89)90699-9

Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions

M. Taglialatela, L. M T Canzoniero, S. Amoroso, A. Fatatis, G. F. Di Renzo, L. Annunziato

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Research output: Contribution to journal › Article

Abstract

Recently, it has been demonstrated that Ca²⁺ entrance into the neuronal cytoplasm can occur upon the activation of 3 different types of specific voltage-dependent channels which can be characterized according to the following criteria: (1) voltage threshold for activation; (2) tendency to inactivation; (3) bivalent cation permeability; and (4) drug sensitivity. In this study we investigated, in tuberoinfundibular dopaminergic (TIDA) hypothalamic neurons, the biochemical and pharmacological properties of Ca²⁺ channels, by comparing the effects of high extracellular concentrations of Ba²⁺ and Ca²⁺ ions on [³H]dopamine (DA) release from TIDA neurons. The results obtained show that extracellular Ba²⁺ ion concentrations dose-dependently (10-20 mM) stimulated [³H]DA release from superfused TIDA neurons and that this effect was prevented by Co²⁺ ions (2 mM). In addition, superfusion of TIDA neurons with a concentration of Ca²⁺ ions equimolar to that by Ba²⁺ ions (20 mM) failed to modify [³H]DA release. The fact that tetraethylammonium (10 mM), a blocker of K⁺ currents in excitable cells, did not mimick the stimulatory action of Ba²⁺ ions on [³H]DA release, seems to exclude that the effect of Ba²⁺ ions was dependent on the inhibition of K⁺ channels in TIDA neurons. The ommission of Ca²⁺ ions from the extracellular medium did not prevent the stimulatory effect on [³H]DA release elicited by elevated concentrations of Ba²⁺ ions, but rather reinforced this effect. Finally, nitredipine (50 μM) did not modify the stimulatory effect of high extracellular Ba²⁺ ions on [³H]DA release from TIDA neurons.

Original language	English
Pages (from-to)	114-120
Number of pages	7
Journal	Brain Research
Volume	488
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(89)90699-9
Publication status	Published - May 29 1989

Fingerprint

Barium

Cobalt

Dopamine

Ions

Dopaminergic Neurons

Neurons

1,4-dihydropyridine

Dopamine Agents

Tetraethylammonium

Cations

Permeability

Cytoplasm

Pharmacology

Keywords

Barium
Calcium
Dihydropyridine
Dopamine release
Tuberoinfundibular dopaminergic neuron
Voltage-dependent Ca channel

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

Cite this

Taglialatela, M., Canzoniero, L. M. T., Amoroso, S., Fatatis, A., Di Renzo, G. F., & Annunziato, L. (1989). Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions. Brain Research, 488(1-2), 114-120. https://doi.org/10.1016/0006-8993(89)90699-9

Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions. / Taglialatela, M.; Canzoniero, L. M T; Amoroso, S.; Fatatis, A.; Di Renzo, G. F.; Annunziato, L.

In: Brain Research, Vol. 488, No. 1-2, 29.05.1989, p. 114-120.

Research output: Contribution to journal › Article

Taglialatela, M, Canzoniero, LMT, Amoroso, S, Fatatis, A, Di Renzo, GF & Annunziato, L 1989, 'Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions', Brain Research, vol. 488, no. 1-2, pp. 114-120. https://doi.org/10.1016/0006-8993(89)90699-9

Taglialatela M, Canzoniero LMT, Amoroso S, Fatatis A, Di Renzo GF, Annunziato L. Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions. Brain Research. 1989 May 29;488(1-2):114-120. https://doi.org/10.1016/0006-8993(89)90699-9

Taglialatela, M. ; Canzoniero, L. M T ; Amoroso, S. ; Fatatis, A. ; Di Renzo, G. F. ; Annunziato, L. / Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions. In: Brain Research. 1989 ; Vol. 488, No. 1-2. pp. 114-120.

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title = "Cobalt-sensitive and dihydropyridine-insensitive stimulation of dopamine release from tuberoinfundibular neurons by high extracellular concentrations of barium ions",

abstract = "Recently, it has been demonstrated that Ca2+ entrance into the neuronal cytoplasm can occur upon the activation of 3 different types of specific voltage-dependent channels which can be characterized according to the following criteria: (1) voltage threshold for activation; (2) tendency to inactivation; (3) bivalent cation permeability; and (4) drug sensitivity. In this study we investigated, in tuberoinfundibular dopaminergic (TIDA) hypothalamic neurons, the biochemical and pharmacological properties of Ca2+ channels, by comparing the effects of high extracellular concentrations of Ba2+ and Ca2+ ions on [3H]dopamine (DA) release from TIDA neurons. The results obtained show that extracellular Ba2+ ion concentrations dose-dependently (10-20 mM) stimulated [3H]DA release from superfused TIDA neurons and that this effect was prevented by Co2+ ions (2 mM). In addition, superfusion of TIDA neurons with a concentration of Ca2+ ions equimolar to that by Ba2+ ions (20 mM) failed to modify [3H]DA release. The fact that tetraethylammonium (10 mM), a blocker of K+ currents in excitable cells, did not mimick the stimulatory action of Ba2+ ions on [3H]DA release, seems to exclude that the effect of Ba2+ ions was dependent on the inhibition of K+ channels in TIDA neurons. The ommission of Ca2+ ions from the extracellular medium did not prevent the stimulatory effect on [3H]DA release elicited by elevated concentrations of Ba2+ ions, but rather reinforced this effect. Finally, nitredipine (50 μM) did not modify the stimulatory effect of high extracellular Ba2+ ions on [3H]DA release from TIDA neurons.",

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AU - Di Renzo, G. F.

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10.1016/0006-8993(89)90699-9