Cocaine potentiates the switch between latency and replication of Epstein-Barr virus in Raji cells

Paolo Di Francesco, Antonella Lisi, Sabrina Rieti, Vanessa Manni, Settimio Grimaldi, Enrico Garaci

Research output: Contribution to journalArticle

Abstract

This paper shows that cocaine amplifies Epstein-Barr virus (EBV) reactivation in Raji cells. Its effect on early viral protein synthesis was maximal when it was added with 12-O-tetradecanoyl phorbol-13-acetate (TPA) plus n-butyrate, but nil when added alone. The enhancing effect of cocaine on early replicative stages of latent EBV was associated with an increase of Ca2+ mobilization induced by the drug and with an induction of cellular protein phosphorylation in chemicals and cocaine-treated Raji cells. Cocaine also acted synergistically with TPA and n-butyrate to induce Z Epstein-Barr replication activator (ZEBRA), a nuclear phosphoprotein responsible for the activation of early viral gene expression. These findings provide the first evidence that cocaine may represent an important cofactor in the reactivation of early stages of latent EBV infection.

Original languageEnglish
Pages (from-to)33-36
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume264
Issue number1
DOIs
Publication statusPublished - Oct 14 1999

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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