Cochlear repair by transplantation of human cord blood CD133+ cells to nod-scid mice made deaf with kanamycin and noise

Roberto P. Revoltella, Sandra Papini, Alfredo Rosellini, Monica Michelini, Valeria Franceschini, Andrea Ciorba, Lucia Bertolaso, Sara Magosso, Stavros Hatzopoulos, Guiscardo Lorito, Pietro Giordano, Edi Simoni, Emanuela Ognio, Michele Cilli, Riccardo Saccardi, Serena Urbani, Rosemary Jeffery, Richard Poulsom, Alessandro Martini

Research output: Contribution to journalArticle

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Abstract

We investigated the fate of human cord blood CD133+ hematopoietic stem cells (HSC) transplanted intravenously (IV) into irradiated nod-scid mice previously made deaf by ototoxic treatment with kanamycin and/or intense noise, to verify whether HSC engraft the cochlea and contribute to inner ear restoration, in vivo. We tested the presence of HLA.DQα1 by PCR, used for traceability of engrafted cells, finding evidence that HSC migrated to various host tissues, including the organ of Corti (OC). By histology, antibody and lectin-staining analysis, we confirmed that HSC IV transplantation in mice previously damaged by ototoxic agents correlated with the repair process and stimulation ex novo of morphological recovery in the inner ear, while the cochlea of control oto-injured, nontransplanted mice remained seriously damaged. Dual color FISH analysis also provided evidence of positive engraftment in the inner ear and in various mouse tissues, also revealing small numbers of heterokaryons, probably derived from fusion of donor with endogenous cells, for up to 2 months following transplantation. These observations offer the first evidence that transplanted human HSC migrating to the inner ear of oto-injured mice may provide conditions for the resumption of deafened cochlea, emerging as a potential strategy for inner ear rehabilitation.

LanguageEnglish
Pages665-678
Number of pages14
JournalCell Transplantation
Volume17
Issue number6
DOIs
StatePublished - 2008

Fingerprint

Kanamycin
Cochlea
Inner Ear
Stem cells
Fetal Blood
Noise
Blood Cells
Hematopoietic Stem Cells
Repair
Blood
Transplantation
Tissue
Organ of Corti
Histology
Hematopoietic Stem Cell Transplantation
Lectins
Antibodies
Patient rehabilitation
Restoration
Fusion reactions

Keywords

  • Cochlea repair
  • Deafness
  • Organ of corti
  • Stem cell
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Revoltella, R. P., Papini, S., Rosellini, A., Michelini, M., Franceschini, V., Ciorba, A., ... Martini, A. (2008). Cochlear repair by transplantation of human cord blood CD133+ cells to nod-scid mice made deaf with kanamycin and noise. Cell Transplantation, 17(6), 665-678. DOI: 10.3727/096368908786092685

Cochlear repair by transplantation of human cord blood CD133+ cells to nod-scid mice made deaf with kanamycin and noise. / Revoltella, Roberto P.; Papini, Sandra; Rosellini, Alfredo; Michelini, Monica; Franceschini, Valeria; Ciorba, Andrea; Bertolaso, Lucia; Magosso, Sara; Hatzopoulos, Stavros; Lorito, Guiscardo; Giordano, Pietro; Simoni, Edi; Ognio, Emanuela; Cilli, Michele; Saccardi, Riccardo; Urbani, Serena; Jeffery, Rosemary; Poulsom, Richard; Martini, Alessandro.

In: Cell Transplantation, Vol. 17, No. 6, 2008, p. 665-678.

Research output: Contribution to journalArticle

Revoltella, RP, Papini, S, Rosellini, A, Michelini, M, Franceschini, V, Ciorba, A, Bertolaso, L, Magosso, S, Hatzopoulos, S, Lorito, G, Giordano, P, Simoni, E, Ognio, E, Cilli, M, Saccardi, R, Urbani, S, Jeffery, R, Poulsom, R & Martini, A 2008, 'Cochlear repair by transplantation of human cord blood CD133+ cells to nod-scid mice made deaf with kanamycin and noise' Cell Transplantation, vol. 17, no. 6, pp. 665-678. DOI: 10.3727/096368908786092685
Revoltella RP, Papini S, Rosellini A, Michelini M, Franceschini V, Ciorba A et al. Cochlear repair by transplantation of human cord blood CD133+ cells to nod-scid mice made deaf with kanamycin and noise. Cell Transplantation. 2008;17(6):665-678. Available from, DOI: 10.3727/096368908786092685
Revoltella, Roberto P. ; Papini, Sandra ; Rosellini, Alfredo ; Michelini, Monica ; Franceschini, Valeria ; Ciorba, Andrea ; Bertolaso, Lucia ; Magosso, Sara ; Hatzopoulos, Stavros ; Lorito, Guiscardo ; Giordano, Pietro ; Simoni, Edi ; Ognio, Emanuela ; Cilli, Michele ; Saccardi, Riccardo ; Urbani, Serena ; Jeffery, Rosemary ; Poulsom, Richard ; Martini, Alessandro. / Cochlear repair by transplantation of human cord blood CD133+ cells to nod-scid mice made deaf with kanamycin and noise. In: Cell Transplantation. 2008 ; Vol. 17, No. 6. pp. 665-678
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