Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016

Cecilia Fazio, Arianna Neri, Paola Vacca, Andrea Ciammaruconi, Milena Arghittu, Anna Maria Barbui, Caterina Vocale, Paola Bernaschi, Patrizia Isola, Irene Alessandra Galanti, Antonella Mencacci, Rosella De Nittis, Maria Chironna, Anna Giammanco, Elisabetta Pagani, Alessandro Bisbano, Paola Stefanelli

Research output: Contribution to journalArticle

Abstract

In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in the period from 2013 to 2016, an increase in serogroup W Neisseria meningitidis (MenW) was observed. This study intends to define the main characteristics of 63 MenW isolates responsible of invasive meningococcal disease (IMD) in Italy from 2000 to 2016. We performed whole genome sequencing on bacterial isolates or single gene sequencing on culture-negative samples to evaluate molecular heterogeneity. Our main finding was the cocirculation of the Hajj and the South American sublineages belonging to MenW/clonal complex (cc)11, which gradually surpassed the MenW/cc22 in Italy. All MenW/cc11 isolates were fully susceptible to cefotaxime, ceftriaxone, ciprofloxacin, penicillin G and rifampicin. We identified the full-length NadA protein variant 2/3, present in all the MenW/cc11. We also identified the fHbp variant 1, which we found exclusively in the MenW/cc11/Hajj sublineage. Concern about the epidemic potential of MenW/cc11 has increased worldwide since the year 2000. Continued surveillance, supported by genomic characterisation, allows high-resolution tracking of pathogen dissemination and the detection of epidemic-associated strains.

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Neisseria meningitidis
Italy
Penicillin G
Cefotaxime
Ceftriaxone
Rifampin
Ciprofloxacin
Genome
Genes
Serogroup
Proteins

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Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016. / Fazio, Cecilia; Neri, Arianna; Vacca, Paola; Ciammaruconi, Andrea; Arghittu, Milena; Barbui, Anna Maria; Vocale, Caterina; Bernaschi, Paola; Isola, Patrizia; Galanti, Irene Alessandra; Mencacci, Antonella; De Nittis, Rosella; Chironna, Maria; Giammanco, Anna; Pagani, Elisabetta; Bisbano, Alessandro; Stefanelli, Paola.

In: Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, Vol. 24, No. 4, 01.2019.

Research output: Contribution to journalArticle

Fazio, Cecilia ; Neri, Arianna ; Vacca, Paola ; Ciammaruconi, Andrea ; Arghittu, Milena ; Barbui, Anna Maria ; Vocale, Caterina ; Bernaschi, Paola ; Isola, Patrizia ; Galanti, Irene Alessandra ; Mencacci, Antonella ; De Nittis, Rosella ; Chironna, Maria ; Giammanco, Anna ; Pagani, Elisabetta ; Bisbano, Alessandro ; Stefanelli, Paola. / Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016. In: Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin. 2019 ; Vol. 24, No. 4.
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title = "Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016",
abstract = "In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in the period from 2013 to 2016, an increase in serogroup W Neisseria meningitidis (MenW) was observed. This study intends to define the main characteristics of 63 MenW isolates responsible of invasive meningococcal disease (IMD) in Italy from 2000 to 2016. We performed whole genome sequencing on bacterial isolates or single gene sequencing on culture-negative samples to evaluate molecular heterogeneity. Our main finding was the cocirculation of the Hajj and the South American sublineages belonging to MenW/clonal complex (cc)11, which gradually surpassed the MenW/cc22 in Italy. All MenW/cc11 isolates were fully susceptible to cefotaxime, ceftriaxone, ciprofloxacin, penicillin G and rifampicin. We identified the full-length NadA protein variant 2/3, present in all the MenW/cc11. We also identified the fHbp variant 1, which we found exclusively in the MenW/cc11/Hajj sublineage. Concern about the epidemic potential of MenW/cc11 has increased worldwide since the year 2000. Continued surveillance, supported by genomic characterisation, allows high-resolution tracking of pathogen dissemination and the detection of epidemic-associated strains.",
author = "Cecilia Fazio and Arianna Neri and Paola Vacca and Andrea Ciammaruconi and Milena Arghittu and Barbui, {Anna Maria} and Caterina Vocale and Paola Bernaschi and Patrizia Isola and Galanti, {Irene Alessandra} and Antonella Mencacci and {De Nittis}, Rosella and Maria Chironna and Anna Giammanco and Elisabetta Pagani and Alessandro Bisbano and Paola Stefanelli",
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T1 - Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016

AU - Fazio, Cecilia

AU - Neri, Arianna

AU - Vacca, Paola

AU - Ciammaruconi, Andrea

AU - Arghittu, Milena

AU - Barbui, Anna Maria

AU - Vocale, Caterina

AU - Bernaschi, Paola

AU - Isola, Patrizia

AU - Galanti, Irene Alessandra

AU - Mencacci, Antonella

AU - De Nittis, Rosella

AU - Chironna, Maria

AU - Giammanco, Anna

AU - Pagani, Elisabetta

AU - Bisbano, Alessandro

AU - Stefanelli, Paola

PY - 2019/1

Y1 - 2019/1

N2 - In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in the period from 2013 to 2016, an increase in serogroup W Neisseria meningitidis (MenW) was observed. This study intends to define the main characteristics of 63 MenW isolates responsible of invasive meningococcal disease (IMD) in Italy from 2000 to 2016. We performed whole genome sequencing on bacterial isolates or single gene sequencing on culture-negative samples to evaluate molecular heterogeneity. Our main finding was the cocirculation of the Hajj and the South American sublineages belonging to MenW/clonal complex (cc)11, which gradually surpassed the MenW/cc22 in Italy. All MenW/cc11 isolates were fully susceptible to cefotaxime, ceftriaxone, ciprofloxacin, penicillin G and rifampicin. We identified the full-length NadA protein variant 2/3, present in all the MenW/cc11. We also identified the fHbp variant 1, which we found exclusively in the MenW/cc11/Hajj sublineage. Concern about the epidemic potential of MenW/cc11 has increased worldwide since the year 2000. Continued surveillance, supported by genomic characterisation, allows high-resolution tracking of pathogen dissemination and the detection of epidemic-associated strains.

AB - In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in the period from 2013 to 2016, an increase in serogroup W Neisseria meningitidis (MenW) was observed. This study intends to define the main characteristics of 63 MenW isolates responsible of invasive meningococcal disease (IMD) in Italy from 2000 to 2016. We performed whole genome sequencing on bacterial isolates or single gene sequencing on culture-negative samples to evaluate molecular heterogeneity. Our main finding was the cocirculation of the Hajj and the South American sublineages belonging to MenW/clonal complex (cc)11, which gradually surpassed the MenW/cc22 in Italy. All MenW/cc11 isolates were fully susceptible to cefotaxime, ceftriaxone, ciprofloxacin, penicillin G and rifampicin. We identified the full-length NadA protein variant 2/3, present in all the MenW/cc11. We also identified the fHbp variant 1, which we found exclusively in the MenW/cc11/Hajj sublineage. Concern about the epidemic potential of MenW/cc11 has increased worldwide since the year 2000. Continued surveillance, supported by genomic characterisation, allows high-resolution tracking of pathogen dissemination and the detection of epidemic-associated strains.

U2 - 10.2807/1560-7917.ES.2019.24.4.1800183

DO - 10.2807/1560-7917.ES.2019.24.4.1800183

M3 - Article

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JO - Eurosurveillance

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SN - 1560-7917

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