Cocoa protective effects against abnormal fat storage and oxidative stress induced by a high-fat diet involve PPARα signalling activation

Marco Fidaleo, Anna Fracassi, Antonio Zuorro, Roberto Lavecchia, Sandra Moreno, Claudia Sartori

Research output: Contribution to journalArticle


A high-fat (HF) diet increases lipid storage and oxidative stress in mouse liver and this process seems to be mediated by Peroxisome Proliferator-Activated Receptor α (PPARα). In this study we evaluated the protective effect of cocoa against hepatic steatosis induced by a HF diet. The HF diet down-regulated PPARα expression and turned off PPARα-signalling, deregulated the β-oxidation (β-Ox) system and catalase (CAT) activity, increased fat storage, reduced expression of enzymatic activity involved in oxidative defence in the liver and doubled the weight gain per calorie consumed compared to animals under the normal diet. In contrast, cocoa improved hepatic β-Ox, activated PPARα-signalling and up-regulated both gene and protein expression of SOD1. Moreover, when co-administered with the HF diet, cocoa treatment counteracted lipid storage in the liver, improved the lipid-metabolizing activity and oxidative stress defences and normalized the weight gain per calorie consumed.

Original languageEnglish
Pages (from-to)2931-2939
Number of pages9
JournalFood and Function
Issue number11
Publication statusPublished - Nov 1 2014


ASJC Scopus subject areas

  • Food Science
  • Medicine(all)

Cite this