Coding sequence and intron-exon junctions of the c-myb gene are intact in the chronic phase and blast crisis stages of chronic myeloid leukemia patients

R. Bussolari, O. Candini, D. Colomer, F. Corradini, C. Guerzoni, S. A. Mariani, S. Cattelani, C. Silvestri, L. Pecorari, I. Iacobucci, S. Soverini, T. Fasano, G. Martinelli, F. Cervantes, B. Calabretta

Research output: Contribution to journalArticle

Abstract

The c-myb gene encodes a transcription factor required for proliferation, differentiation and survival of normal and leukemic hematopoietic cells. c-Myb has a longer half-life in BCR/ABL-expressing than in normal cells, a feature which depends, in part, on PI-3K/Akt-dependent regulation of proteins interacting with the leucine zipper/negative regulatory region of c-Myb. Thus, we asked whether the stability of c-Myb in leukemic cells might be enhanced by mutations interfering with its degradation. We analyzed the c-myb gene in 133 chronic myeloid leukemia (CML) patients in chronic phase and/or blast crisis by denaturing-high performance liquid chromatography (D-HPLC) and sequence analysis of PCR products corresponding to the entire coding sequence and each exon-intron boundary. No mutations were found. We found four single nucleotide polymorphisms (SNPs) and identified an alternatively spliced transcript lacking exon 5, but SNPs frequency and expression of the alternatively spliced transcript were identical in normal and CML cells. Thus, the enhanced stability of c-Myb in CML blast crisis cells and perhaps in other types of leukemia is not caused by a genetic mechanism.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalLeukemia Research
Volume31
Issue number2
DOIs
Publication statusPublished - Feb 2007

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Keywords

  • c-Myb
  • Chronic myeloid leukemia
  • Denaturing-high performance liquid chromatography
  • Proto-oncogene

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Bussolari, R., Candini, O., Colomer, D., Corradini, F., Guerzoni, C., Mariani, S. A., Cattelani, S., Silvestri, C., Pecorari, L., Iacobucci, I., Soverini, S., Fasano, T., Martinelli, G., Cervantes, F., & Calabretta, B. (2007). Coding sequence and intron-exon junctions of the c-myb gene are intact in the chronic phase and blast crisis stages of chronic myeloid leukemia patients. Leukemia Research, 31(2), 163-167. https://doi.org/10.1016/j.leukres.2006.05.007