Coexistence of anti-RNA polymerase III and anti-U1RNP antibodies in patients with systemic lupus erythematosus

Two cases without features of scleroderma

M. Satoh, M. Vazquez-Del Mercado, M. E. Krzyszczak, Y. Li, A. Ceribelli, R. W. Burlingame, T. T. Webb, E. S. Sobel, W. H. Reeves, Ekl Chan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Anti-RNA polymerase III (RNAP III) antibodies are highly specific for scleroderma (SSc) and associated with diffuse SSc and renal crisis. Coexistence of anti-RNAP III and other SSc autoantibodies is rarely documented. We report three cases with coexisting anti-RNAP III and anti-U1RNP. Autoantibodies in 3829 sera from rheumatology clinics were screened by immunoprecipitation. Anti-RNAP III-positive sera were also examined by immunofluorescence and anti-RNAP III ELISA. In total, 35 anti-RNAP III-positive sera were identified by immunoprecipitation, in which three had coexisting anti-U1RNP. All three were anti-RNAP III ELISA positive. Two had anti-RNAP I dominant (vs. RNAP III) reactivity and showed strong nucleolar staining. A case with anti-U1/U2RNP (U2RNP dominant) had systemic lupus erythematosus (SLE)-SSc overlap syndrome; however, the remaining two cases had SLE without signs of SSc. All three cases of anti-RNAP III + U1RNP fulfilled ACR SLE criteria but none in the group with anti-RNAP III alone (p = 0.0002). In contrast, only one case in the former group had sclerodermatous skin changes and Raynaud's phenomenon, vs. 92% with scleroderma in the latter (p <0.05). Although anti-RNAP III is highly specific for SSc, cases with coexisting anti-U1RNP are not so uncommon among anti-RNAP III positives (8%, 3/35) and may be SLE without features of SSc.

Original languageEnglish
Pages (from-to)68-74
Number of pages7
JournalLupus
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

RNA Polymerase III
Systemic Lupus Erythematosus
Anti-Idiotypic Antibodies
Immunoprecipitation
Autoantibodies
Serum
Enzyme-Linked Immunosorbent Assay
Diffuse Scleroderma
Raynaud Disease
Rheumatology
Fluorescent Antibody Technique

Keywords

  • anti-RNA polymerase III antibodies
  • anti-U1RNP antibodies
  • autoantibodies
  • scleroderma

ASJC Scopus subject areas

  • Rheumatology

Cite this

Coexistence of anti-RNA polymerase III and anti-U1RNP antibodies in patients with systemic lupus erythematosus : Two cases without features of scleroderma. / Satoh, M.; Vazquez-Del Mercado, M.; Krzyszczak, M. E.; Li, Y.; Ceribelli, A.; Burlingame, R. W.; Webb, T. T.; Sobel, E. S.; Reeves, W. H.; Chan, Ekl.

In: Lupus, Vol. 21, No. 1, 01.2012, p. 68-74.

Research output: Contribution to journalArticle

Satoh, M, Vazquez-Del Mercado, M, Krzyszczak, ME, Li, Y, Ceribelli, A, Burlingame, RW, Webb, TT, Sobel, ES, Reeves, WH & Chan, E 2012, 'Coexistence of anti-RNA polymerase III and anti-U1RNP antibodies in patients with systemic lupus erythematosus: Two cases without features of scleroderma', Lupus, vol. 21, no. 1, pp. 68-74. https://doi.org/10.1177/0961203311422712
Satoh, M. ; Vazquez-Del Mercado, M. ; Krzyszczak, M. E. ; Li, Y. ; Ceribelli, A. ; Burlingame, R. W. ; Webb, T. T. ; Sobel, E. S. ; Reeves, W. H. ; Chan, Ekl. / Coexistence of anti-RNA polymerase III and anti-U1RNP antibodies in patients with systemic lupus erythematosus : Two cases without features of scleroderma. In: Lupus. 2012 ; Vol. 21, No. 1. pp. 68-74.
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abstract = "Anti-RNA polymerase III (RNAP III) antibodies are highly specific for scleroderma (SSc) and associated with diffuse SSc and renal crisis. Coexistence of anti-RNAP III and other SSc autoantibodies is rarely documented. We report three cases with coexisting anti-RNAP III and anti-U1RNP. Autoantibodies in 3829 sera from rheumatology clinics were screened by immunoprecipitation. Anti-RNAP III-positive sera were also examined by immunofluorescence and anti-RNAP III ELISA. In total, 35 anti-RNAP III-positive sera were identified by immunoprecipitation, in which three had coexisting anti-U1RNP. All three were anti-RNAP III ELISA positive. Two had anti-RNAP I dominant (vs. RNAP III) reactivity and showed strong nucleolar staining. A case with anti-U1/U2RNP (U2RNP dominant) had systemic lupus erythematosus (SLE)-SSc overlap syndrome; however, the remaining two cases had SLE without signs of SSc. All three cases of anti-RNAP III + U1RNP fulfilled ACR SLE criteria but none in the group with anti-RNAP III alone (p = 0.0002). In contrast, only one case in the former group had sclerodermatous skin changes and Raynaud's phenomenon, vs. 92{\%} with scleroderma in the latter (p <0.05). Although anti-RNAP III is highly specific for SSc, cases with coexisting anti-U1RNP are not so uncommon among anti-RNAP III positives (8{\%}, 3/35) and may be SLE without features of SSc.",
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AB - Anti-RNA polymerase III (RNAP III) antibodies are highly specific for scleroderma (SSc) and associated with diffuse SSc and renal crisis. Coexistence of anti-RNAP III and other SSc autoantibodies is rarely documented. We report three cases with coexisting anti-RNAP III and anti-U1RNP. Autoantibodies in 3829 sera from rheumatology clinics were screened by immunoprecipitation. Anti-RNAP III-positive sera were also examined by immunofluorescence and anti-RNAP III ELISA. In total, 35 anti-RNAP III-positive sera were identified by immunoprecipitation, in which three had coexisting anti-U1RNP. All three were anti-RNAP III ELISA positive. Two had anti-RNAP I dominant (vs. RNAP III) reactivity and showed strong nucleolar staining. A case with anti-U1/U2RNP (U2RNP dominant) had systemic lupus erythematosus (SLE)-SSc overlap syndrome; however, the remaining two cases had SLE without signs of SSc. All three cases of anti-RNAP III + U1RNP fulfilled ACR SLE criteria but none in the group with anti-RNAP III alone (p = 0.0002). In contrast, only one case in the former group had sclerodermatous skin changes and Raynaud's phenomenon, vs. 92% with scleroderma in the latter (p <0.05). Although anti-RNAP III is highly specific for SSc, cases with coexisting anti-U1RNP are not so uncommon among anti-RNAP III positives (8%, 3/35) and may be SLE without features of SSc.

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