Coexpression of aspartic proteinases and human leukocyte antigen-Dr in human transplanted lung

Eloisa Arbustini, Patrizia Morbini, Marta Diegoli, Maurizia Grasso, Roberta Fasani, Patrizio Vitulo, Roberto Fiocca, Paolo Cremaschi, Gino Volpato, Luigi Martinelli, Mario Viganò, I. Michael Samloff, Enrico Solcia

Research output: Contribution to journalArticle

Abstract

Aspartic proteinases have recently been shown to be implicated in antigen processing. We explored the expression of two aspartic proteinases, cathepsin E and D, and of human leukocyte antigen-DR (HLA-DR) molecules in a consecutive series of 80 transbronchial biopsies from transplanted lungs. For controls, we studied five normal donor lungs (not suitable for transplantation on account of thoracic trauma) and macroscopically normal areas of three cancer-affected lungs. Two of the five unsuitable donor lungs showed minimal inflammatory changes. Macroscopically normal samples from the three cancerous lungs showed mild and focal inflammatory infiltrates. In histologically normal lungs, HLA-DR expression was limited to professional antigen presenting cells. Macroscopically normal lung samples with minimal inflammatory changes from both donor and cancer lungs showed variable HLA-DR expression by alveolar and bronchial epithelial cells and by endothelial cells. All transplanted lung biopsies showed HLA-DR expression by epithelial (alveolar and bronchial) and endothelial cells, with a trend for increased positivity in acute rejection. Cathepsin E was restricted to Clara and to rare bronchus-associated lymphoid tissue-related epithelial cells in histologically normal lung samples, whereas minimal de novo cathepsin E expression by rare alveolar pneumocytes was noted in control lung samples exhibiting minimal inflammatory changes. In all transplanted lung biopsies, cathepsin E was diffusely expressed de novo by hyperplastic alveolar epithelial cells, regardless of the presence or degree of rejection. Cathepsin D was expressed only by alveolar macrophages and by ciliated bronchial cells of normal, minimally inflamed, and transplanted lungs. In transplanted lung, Clara cells and several hyperplastic alveolar pneumocytes coexpressed HLA-DR and cathepsin E, whereas all alveolar macrophages and a few ciliated cells coexpressed cathepsin D and HLA-DR. The present investigation suggests that the de novo expression of cathepsin E and HLA-DR by hyperplastic alveolar pneumocytes of transplanted lung may be crucial for antigen processing and presentation to recipient competent T cells, and thus for the triggering of the immune-inflammatory cascade that leads to rejection.

Original languageEnglish
Pages (from-to)310-321
Number of pages12
JournalAmerican Journal of Pathology
Volume145
Issue number2
Publication statusPublished - Aug 1994

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Aspartic Acid Proteases
HLA Antigens
Cathepsin E
Lung
Alveolar Epithelial Cells
Cathepsin D
Antigen Presentation
Alveolar Macrophages
Biopsy
Lung Neoplasms
Endothelial Cells
Lymphoid Tissue
Antigen-Presenting Cells
Bronchi

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Coexpression of aspartic proteinases and human leukocyte antigen-Dr in human transplanted lung. / Arbustini, Eloisa; Morbini, Patrizia; Diegoli, Marta; Grasso, Maurizia; Fasani, Roberta; Vitulo, Patrizio; Fiocca, Roberto; Cremaschi, Paolo; Volpato, Gino; Martinelli, Luigi; Viganò, Mario; Samloff, I. Michael; Solcia, Enrico.

In: American Journal of Pathology, Vol. 145, No. 2, 08.1994, p. 310-321.

Research output: Contribution to journalArticle

Arbustini, E, Morbini, P, Diegoli, M, Grasso, M, Fasani, R, Vitulo, P, Fiocca, R, Cremaschi, P, Volpato, G, Martinelli, L, Viganò, M, Samloff, IM & Solcia, E 1994, 'Coexpression of aspartic proteinases and human leukocyte antigen-Dr in human transplanted lung', American Journal of Pathology, vol. 145, no. 2, pp. 310-321.
Arbustini, Eloisa ; Morbini, Patrizia ; Diegoli, Marta ; Grasso, Maurizia ; Fasani, Roberta ; Vitulo, Patrizio ; Fiocca, Roberto ; Cremaschi, Paolo ; Volpato, Gino ; Martinelli, Luigi ; Viganò, Mario ; Samloff, I. Michael ; Solcia, Enrico. / Coexpression of aspartic proteinases and human leukocyte antigen-Dr in human transplanted lung. In: American Journal of Pathology. 1994 ; Vol. 145, No. 2. pp. 310-321.
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abstract = "Aspartic proteinases have recently been shown to be implicated in antigen processing. We explored the expression of two aspartic proteinases, cathepsin E and D, and of human leukocyte antigen-DR (HLA-DR) molecules in a consecutive series of 80 transbronchial biopsies from transplanted lungs. For controls, we studied five normal donor lungs (not suitable for transplantation on account of thoracic trauma) and macroscopically normal areas of three cancer-affected lungs. Two of the five unsuitable donor lungs showed minimal inflammatory changes. Macroscopically normal samples from the three cancerous lungs showed mild and focal inflammatory infiltrates. In histologically normal lungs, HLA-DR expression was limited to professional antigen presenting cells. Macroscopically normal lung samples with minimal inflammatory changes from both donor and cancer lungs showed variable HLA-DR expression by alveolar and bronchial epithelial cells and by endothelial cells. All transplanted lung biopsies showed HLA-DR expression by epithelial (alveolar and bronchial) and endothelial cells, with a trend for increased positivity in acute rejection. Cathepsin E was restricted to Clara and to rare bronchus-associated lymphoid tissue-related epithelial cells in histologically normal lung samples, whereas minimal de novo cathepsin E expression by rare alveolar pneumocytes was noted in control lung samples exhibiting minimal inflammatory changes. In all transplanted lung biopsies, cathepsin E was diffusely expressed de novo by hyperplastic alveolar epithelial cells, regardless of the presence or degree of rejection. Cathepsin D was expressed only by alveolar macrophages and by ciliated bronchial cells of normal, minimally inflamed, and transplanted lungs. In transplanted lung, Clara cells and several hyperplastic alveolar pneumocytes coexpressed HLA-DR and cathepsin E, whereas all alveolar macrophages and a few ciliated cells coexpressed cathepsin D and HLA-DR. The present investigation suggests that the de novo expression of cathepsin E and HLA-DR by hyperplastic alveolar pneumocytes of transplanted lung may be crucial for antigen processing and presentation to recipient competent T cells, and thus for the triggering of the immune-inflammatory cascade that leads to rejection.",
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AU - Vitulo, Patrizio

AU - Fiocca, Roberto

AU - Cremaschi, Paolo

AU - Volpato, Gino

AU - Martinelli, Luigi

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N2 - Aspartic proteinases have recently been shown to be implicated in antigen processing. We explored the expression of two aspartic proteinases, cathepsin E and D, and of human leukocyte antigen-DR (HLA-DR) molecules in a consecutive series of 80 transbronchial biopsies from transplanted lungs. For controls, we studied five normal donor lungs (not suitable for transplantation on account of thoracic trauma) and macroscopically normal areas of three cancer-affected lungs. Two of the five unsuitable donor lungs showed minimal inflammatory changes. Macroscopically normal samples from the three cancerous lungs showed mild and focal inflammatory infiltrates. In histologically normal lungs, HLA-DR expression was limited to professional antigen presenting cells. Macroscopically normal lung samples with minimal inflammatory changes from both donor and cancer lungs showed variable HLA-DR expression by alveolar and bronchial epithelial cells and by endothelial cells. All transplanted lung biopsies showed HLA-DR expression by epithelial (alveolar and bronchial) and endothelial cells, with a trend for increased positivity in acute rejection. Cathepsin E was restricted to Clara and to rare bronchus-associated lymphoid tissue-related epithelial cells in histologically normal lung samples, whereas minimal de novo cathepsin E expression by rare alveolar pneumocytes was noted in control lung samples exhibiting minimal inflammatory changes. In all transplanted lung biopsies, cathepsin E was diffusely expressed de novo by hyperplastic alveolar epithelial cells, regardless of the presence or degree of rejection. Cathepsin D was expressed only by alveolar macrophages and by ciliated bronchial cells of normal, minimally inflamed, and transplanted lungs. In transplanted lung, Clara cells and several hyperplastic alveolar pneumocytes coexpressed HLA-DR and cathepsin E, whereas all alveolar macrophages and a few ciliated cells coexpressed cathepsin D and HLA-DR. The present investigation suggests that the de novo expression of cathepsin E and HLA-DR by hyperplastic alveolar pneumocytes of transplanted lung may be crucial for antigen processing and presentation to recipient competent T cells, and thus for the triggering of the immune-inflammatory cascade that leads to rejection.

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