Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells

Nicola Gagliani, Chiara F. Magnani, Samuel Huber, Monica E. Gianolini, Mauro Pala, Paula Licona-Limon, Binggege Guo, De'Broski R. Herbert, Alessandro Bulfone, Filippo Trentini, Clelia Di Serio, Rosa Bacchetta, Marco Andreani, Leonie Brockmann, Silvia Gregori, Richard A. Flavell, Maria Grazia Roncarolo

Research output: Contribution to journalArticlepeer-review

Abstract

CD4+ type 1 T regulatory (Tr1) cells are induced in the periphery and have a pivotal role in promoting and maintaining tolerance. The absence of surface markers that uniquely identify Tr1 cells has limited their study and clinical applications. By gene expression profiling of human Tr1 cell clones, we identified the surface markers CD49b and lymphocyte activation gene 3 (LAG-3) as being stably and selectively coexpressed on mouse and human Tr1 cells. We showed the specificity of these markers in mouse models of intestinal inflammation and helminth infection and in the peripheral blood of healthy volunteers. The coexpression of CD49b and LAG-3 enables the isolation of highly suppressive human Tr1 cells from in vitro anergized cultures and allows the tracking of Tr1 cells in the peripheral blood of subjects who developed tolerance after allogeneic hematopoietic stem cell transplantation. The use of these markers makes it feasible to track Tr1 cells in vivo and purify Tr1 cells for cell therapy to induce or restore tolerance in subjects with immune-mediated diseases.

Original languageEnglish
Pages (from-to)739-746
Number of pages8
JournalNature Medicine
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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