Cofactors influence the biological properties of infectious recombinant prions.

Natalia Fernández-Borges, Michele Angelo Di Bari, Hasier Eraña, Laura Pirisinu, Beatriz Parra, Saioa R. Elezgarai, Ilaria Vanni, Raphael López-Moreno, Gabriele Vaccari, Vanessa Venegas, Jorge M. Charco, David Gil, Chafik Harrathi, Claudia D'Agostino, Umberto Agrimi, Tomás Mayoral, Jesús R. Requena, Romolo Nonno, Joaquín Castilla

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Prion diseases are caused by a misfolding of the cellular prion protein (PrP) to a pathogenic isoform named PrPSc. Prions exist as strains, which are characterized by specific pathological and biochemical properties likely encoded in the three-dimensional structure of PrPSc. However, whether cofactors determine these different PrPSc conformations and how this relates to their specific biological properties is largely unknown. To understand how different cofactors modulate prion strain generation and selection, Protein Misfolding Cyclic Amplification was used to create a diversity of infectious recombinant prion strains by propagation in the presence of brain homogenate. Brain homogenate is known to contain these mentioned cofactors, whose identity is only partially known, and which facilitate conversion of PrPC to PrPSc. We thus obtained a mix of distinguishable infectious prion strains. Subsequently, we replaced brain homogenate, by different polyanionic cofactors that were able to drive the evolution of mixed prion populations toward specific strains. Thus, our results show that a variety of infectious recombinant prions can be generated in vitro and that their specific type of conformation, i.e., the strain, is dependent on the cofactors available during the propagation process. These observations have significant implications for understanding the pathogenesis of prion diseases and their ability to replicate in different tissues and hosts. Importantly, these considerations might apply to other neurodegenerative diseases for which different conformations of misfolded proteins have been described.
Original languageEnglish
JournalActa Neuropathologica
DOIs
Publication statusE-pub ahead of print - Nov 1 2017

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Prions
Prion Diseases
Brain
Protein Conformation
Neurodegenerative Diseases
Protein Isoforms
Population
Proteins

Keywords

  • Cofactors
  • In vitro propagation
  • Infectious recombinant prions
  • PMCA
  • Prion strains
  • TSE

Cite this

Cofactors influence the biological properties of infectious recombinant prions. / Fernández-Borges, Natalia; Di Bari, Michele Angelo; Eraña, Hasier; Pirisinu, Laura; Parra, Beatriz; Elezgarai, Saioa R.; Vanni, Ilaria; López-Moreno, Raphael; Vaccari, Gabriele; Venegas, Vanessa; Charco, Jorge M.; Gil, David; Harrathi, Chafik; D'Agostino, Claudia; Agrimi, Umberto; Mayoral, Tomás; Requena, Jesús R.; Nonno, Romolo; Castilla, Joaquín.

In: Acta Neuropathologica, 01.11.2017.

Research output: Contribution to journalArticle

Fernández-Borges, N, Di Bari, MA, Eraña, H, Pirisinu, L, Parra, B, Elezgarai, SR, Vanni, I, López-Moreno, R, Vaccari, G, Venegas, V, Charco, JM, Gil, D, Harrathi, C, D'Agostino, C, Agrimi, U, Mayoral, T, Requena, JR, Nonno, R & Castilla, J 2017, 'Cofactors influence the biological properties of infectious recombinant prions.', Acta Neuropathologica. https://doi.org/10.1007/s00401-017-1782-y
Fernández-Borges, Natalia ; Di Bari, Michele Angelo ; Eraña, Hasier ; Pirisinu, Laura ; Parra, Beatriz ; Elezgarai, Saioa R. ; Vanni, Ilaria ; López-Moreno, Raphael ; Vaccari, Gabriele ; Venegas, Vanessa ; Charco, Jorge M. ; Gil, David ; Harrathi, Chafik ; D'Agostino, Claudia ; Agrimi, Umberto ; Mayoral, Tomás ; Requena, Jesús R. ; Nonno, Romolo ; Castilla, Joaquín. / Cofactors influence the biological properties of infectious recombinant prions. In: Acta Neuropathologica. 2017.
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AU - Di Bari, Michele Angelo

AU - Eraña, Hasier

AU - Pirisinu, Laura

AU - Parra, Beatriz

AU - Elezgarai, Saioa R.

AU - Vanni, Ilaria

AU - López-Moreno, Raphael

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AU - Venegas, Vanessa

AU - Charco, Jorge M.

AU - Gil, David

AU - Harrathi, Chafik

AU - D'Agostino, Claudia

AU - Agrimi, Umberto

AU - Mayoral, Tomás

AU - Requena, Jesús R.

AU - Nonno, Romolo

AU - Castilla, Joaquín

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