Cold exposure affects carbohydrates and lipid metabolism, and induces Hog1p phosphorylation in Dekkera bruxellensis strain CBS 2499

Silvia Galafassi, Marco Toscano, Ileana Vigentini, Paolo Zambelli, Paolo Simonetti, Roberto Foschino, Concetta Compagno

Research output: Contribution to journalArticle

Abstract

Dekkera bruxellensis is a yeast known to affect the quality of wine and beer. This species, due to its high ethanol and acid tolerance, has been reported also to compete with Saccharomyces cerevisiae in distilleries producing fuel ethanol. In order to understand how this species responds when exposed to low temperatures, some mechanisms like synthesis and accumulation of intracellular metabolites, changes in lipid composition and activation of the HOG-MAPK pathway were investigated in the genome sequenced strain CBS 2499. We show that cold stress caused intracellular accumulation of glycogen, but did not induce accumulation of trehalose and glycerol. The cellular fatty acid composition changed after the temperature downshift, and a significant increase of palmitoleic acid was observed. RT-PCR analysis revealed that OLE1 encoding for Δ9-fatty acid desaturase was up-regulated, whereas TPS1 and INO1 didn’t show changes in their expression. In D. bruxellensis Hog1p was activated by phosphorylation, as described in S. cerevisiae, highlighting a conserved role of the HOG-MAP kinase signaling pathway in cold stress response.

Original languageEnglish
Pages (from-to)1145-1153
Number of pages9
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume107
Issue number5
DOIs
Publication statusPublished - May 1 2015

Keywords

  • Beer
  • Cold stress
  • D. bruxellensis
  • Ethanol production
  • Wine
  • Yeast

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Medicine(all)

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