Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load

Francesca Sidoti, Antonio Piralla, Cristina Costa, Maria Luisa Scarasciulli, Agata Calvario, Pier Giulio Conaldi, Pierpaolo Paba, Carlo Federico Perno, Aurelia Gaeta, Guido Antonelli, Giuseppe Sodano, Rosaria Santangelo, Maurizio Sanguinetti, Maria Linda Vatteroni, Luisa Barzon, Giorgio Palù, Isabella Abbate, Maria Rosaria Capobianchi, Giulia Piccirilli, Tiziana LazzarottoFausto Baldanti, Rossana Cavallo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present multicentric (n = 11 laboratories) study aimed to identify conversion factors from copies/mL to international units (IU)/mL for the normalization of HCMV DNA load using the first WHO International Standard for HCMV nucleic acid amplification techniques and to enhance interlaboratory agreement of HCMV DNA quantification methods. Study protocols for whole blood and plasma (extraction and amplification) were performed to calculate conversion factors from HCMV DNA copy number to IU. The greatest variability was observed in samples with lower HCMV concentrations (3.0 Log10) in both biological matrices. Overall, 73.1% (206/282) of whole blood and 82.2% (324/394) of plasma samples analyzed fell within an acceptable variation range (±0.5 Log10 difference). An average of 0.64 (range 0.21–1.17) was the conversion factor calculated for the HCMV whole blood panel and 0.82 (range 0.39–2.2) for the HCMV plasma panel.

Original languageEnglish
JournalDiagnostic Microbiology and Infectious Disease
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

DNA
Nucleic Acid Amplification Techniques

Keywords

  • HCMV
  • International standard
  • Multicenter study

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load. / Sidoti, Francesca; Piralla, Antonio; Costa, Cristina; Scarasciulli, Maria Luisa; Calvario, Agata; Conaldi, Pier Giulio; Paba, Pierpaolo; Perno, Carlo Federico; Gaeta, Aurelia; Antonelli, Guido; Sodano, Giuseppe; Santangelo, Rosaria; Sanguinetti, Maurizio; Vatteroni, Maria Linda; Barzon, Luisa; Palù, Giorgio; Abbate, Isabella; Capobianchi, Maria Rosaria; Piccirilli, Giulia; Lazzarotto, Tiziana; Baldanti, Fausto; Cavallo, Rossana.

In: Diagnostic Microbiology and Infectious Disease, 01.01.2019.

Research output: Contribution to journalArticle

Sidoti, Francesca ; Piralla, Antonio ; Costa, Cristina ; Scarasciulli, Maria Luisa ; Calvario, Agata ; Conaldi, Pier Giulio ; Paba, Pierpaolo ; Perno, Carlo Federico ; Gaeta, Aurelia ; Antonelli, Guido ; Sodano, Giuseppe ; Santangelo, Rosaria ; Sanguinetti, Maurizio ; Vatteroni, Maria Linda ; Barzon, Luisa ; Palù, Giorgio ; Abbate, Isabella ; Capobianchi, Maria Rosaria ; Piccirilli, Giulia ; Lazzarotto, Tiziana ; Baldanti, Fausto ; Cavallo, Rossana. / Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load. In: Diagnostic Microbiology and Infectious Disease. 2019.
@article{7a078b5556a540e281e94b3c258ff03b,
title = "Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load",
abstract = "The present multicentric (n = 11 laboratories) study aimed to identify conversion factors from copies/mL to international units (IU)/mL for the normalization of HCMV DNA load using the first WHO International Standard for HCMV nucleic acid amplification techniques and to enhance interlaboratory agreement of HCMV DNA quantification methods. Study protocols for whole blood and plasma (extraction and amplification) were performed to calculate conversion factors from HCMV DNA copy number to IU. The greatest variability was observed in samples with lower HCMV concentrations (3.0 Log10) in both biological matrices. Overall, 73.1{\%} (206/282) of whole blood and 82.2{\%} (324/394) of plasma samples analyzed fell within an acceptable variation range (±0.5 Log10 difference). An average of 0.64 (range 0.21–1.17) was the conversion factor calculated for the HCMV whole blood panel and 0.82 (range 0.39–2.2) for the HCMV plasma panel.",
keywords = "HCMV, International standard, Multicenter study",
author = "Francesca Sidoti and Antonio Piralla and Cristina Costa and Scarasciulli, {Maria Luisa} and Agata Calvario and Conaldi, {Pier Giulio} and Pierpaolo Paba and Perno, {Carlo Federico} and Aurelia Gaeta and Guido Antonelli and Giuseppe Sodano and Rosaria Santangelo and Maurizio Sanguinetti and Vatteroni, {Maria Linda} and Luisa Barzon and Giorgio Pal{\`u} and Isabella Abbate and Capobianchi, {Maria Rosaria} and Giulia Piccirilli and Tiziana Lazzarotto and Fausto Baldanti and Rossana Cavallo",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.diagmicrobio.2019.05.012",
language = "English",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load

AU - Sidoti, Francesca

AU - Piralla, Antonio

AU - Costa, Cristina

AU - Scarasciulli, Maria Luisa

AU - Calvario, Agata

AU - Conaldi, Pier Giulio

AU - Paba, Pierpaolo

AU - Perno, Carlo Federico

AU - Gaeta, Aurelia

AU - Antonelli, Guido

AU - Sodano, Giuseppe

AU - Santangelo, Rosaria

AU - Sanguinetti, Maurizio

AU - Vatteroni, Maria Linda

AU - Barzon, Luisa

AU - Palù, Giorgio

AU - Abbate, Isabella

AU - Capobianchi, Maria Rosaria

AU - Piccirilli, Giulia

AU - Lazzarotto, Tiziana

AU - Baldanti, Fausto

AU - Cavallo, Rossana

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The present multicentric (n = 11 laboratories) study aimed to identify conversion factors from copies/mL to international units (IU)/mL for the normalization of HCMV DNA load using the first WHO International Standard for HCMV nucleic acid amplification techniques and to enhance interlaboratory agreement of HCMV DNA quantification methods. Study protocols for whole blood and plasma (extraction and amplification) were performed to calculate conversion factors from HCMV DNA copy number to IU. The greatest variability was observed in samples with lower HCMV concentrations (3.0 Log10) in both biological matrices. Overall, 73.1% (206/282) of whole blood and 82.2% (324/394) of plasma samples analyzed fell within an acceptable variation range (±0.5 Log10 difference). An average of 0.64 (range 0.21–1.17) was the conversion factor calculated for the HCMV whole blood panel and 0.82 (range 0.39–2.2) for the HCMV plasma panel.

AB - The present multicentric (n = 11 laboratories) study aimed to identify conversion factors from copies/mL to international units (IU)/mL for the normalization of HCMV DNA load using the first WHO International Standard for HCMV nucleic acid amplification techniques and to enhance interlaboratory agreement of HCMV DNA quantification methods. Study protocols for whole blood and plasma (extraction and amplification) were performed to calculate conversion factors from HCMV DNA copy number to IU. The greatest variability was observed in samples with lower HCMV concentrations (3.0 Log10) in both biological matrices. Overall, 73.1% (206/282) of whole blood and 82.2% (324/394) of plasma samples analyzed fell within an acceptable variation range (±0.5 Log10 difference). An average of 0.64 (range 0.21–1.17) was the conversion factor calculated for the HCMV whole blood panel and 0.82 (range 0.39–2.2) for the HCMV plasma panel.

KW - HCMV

KW - International standard

KW - Multicenter study

UR - http://www.scopus.com/inward/record.url?scp=85067187150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067187150&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2019.05.012

DO - 10.1016/j.diagmicrobio.2019.05.012

M3 - Article

AN - SCOPUS:85067187150

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

ER -