Collagen fibre and fibril ultrastructural arrangement of the superficial medial collateral ligament in the human knee

Stefano Zaffagnini, Giulio Maria Marcheggiani Muccioli, Marco Franchi, Beatrice Bacchelli, Alberto Grassi, Patrizia Agati, Marilisa Quaranta, Maurilio Marcacci, Viviana De Pasquale

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The aim of the study was to investigate the collagen fibre ultrastructural arrangement and collagen fibril diameters in the superficial medial collateral ligament (sMCL) in the human knee. Considering sMCL’s distinctive functions at different angles of knee flexion, it was hypothesized a significant difference between the collagen fibril diameters of each portion of the sMCL. Methods: Fourteen sMCL from seven fresh males (by chance because of the availability) cadavers (median age 40 years, range 34–59 years) were harvested within 12 h of death. sMCLs were separated into two orders of regions for analysis. The first order (divisions) was anterior, central and posterior. Thereafter, each division was split into three regions (femoral, intermediate and tibial), generating nine portions. One sMCL from each cadaver was used for transmission electron microscopy (TEM) and morphometric analyses, whereas the contralateral sMCL was processed for light microscopy (LM) or scanning electron microscopy (SEM). Results: LM and SEM analyses showed a complex tridimensional architecture, with the presence of wavy collagen fibres or crimps. TEM analysis showed significant differences in median collagen fibril diameter among portions inside the anterior, central and posterior division of the sMCL (p 

Original languageEnglish
Pages (from-to)3674-3682
Number of pages9
JournalKnee Surgery, Sports Traumatology, Arthroscopy
Volume23
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Keywords

  • Collagen fibre array
  • Crimps
  • Human
  • Knee
  • Medial collateral ligament
  • Ultrastructure

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

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