Abstract
This paper describes the spatial organization of the collagen fibrillar skeleton in pregnant rabbit endometrium at term, employing an alkali/low temperature/maceration technique followed by scanning electron microscopic (SEM) observations. Parallel light microscopic (LM) and transmission electron microscopic (TEM) investigations were made to identify the location and possible changes in the endometrial collagen network. Two different types of NaOH maceration were applied, demonstrating separately: 1) the collagen structures (2N-NaOH maceration removes the cells and basal laminae); and 2) the cellular elements (6N-NaOH maceration removes the collagen fibrils). After 2N-NaOH maceration, the collagen network of the endometrium is seen in a superficial compartment around the glands and a deep compartment situated near the endometrial-myometrial junction. Significant changes are observed only in the superficial compartment. The luminal mucosal surface is characterized by numerous thin projections reducing the uterine cavity which, as a consequence, further appears very irregular and highly convoluted. The subepithelial collagen network is composed of densely packed fibrils with a woven course. It contains many tubular or channel-like invaginations (100- 150 μm in width and 200-300 μm in length) where endometrial glands are located. These invaginations, corresponding to the glandular impressions, are extremely dilated, enlarged and variable in shape. The collagen fibrils are arranged concentrically around the glandular orifices without forming bundles. At the bottom of the spaces between the mucosal projections, small fenestrations (4-8 μm in diameter each) are present. They form small groups of about 10-20 in number and are due to the endometrial blood capillaries. The deep compartment of the endometrial collagen network is little altered, preserving its general lamellar arrangement. The changes in the endometrial collagen skeleton are due to a variety of complex mechanical and hormonal stimuli affecting the uterus during pregnancy. These may be significant for correct implantation, placentation and delivery.
| Original language | English |
|---|---|
| Pages (from-to) | 127-135 |
| Number of pages | 9 |
| Journal | Archives of Histology and Cytology |
| Volume | 59 |
| Issue number | 2 |
| Publication status | Published - May 1996 |
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ASJC Scopus subject areas
- Anatomy
- Histology
Cite this
Collagen fibrillar skeleton in pregnant rabbit endometrium at term : A SEM study after NaOH maceration. / Goranova, Vania; Vizza, Enrico; Correr, Silvia; Heyn, Rosemarie; Motta, Pietro M.
In: Archives of Histology and Cytology, Vol. 59, No. 2, 05.1996, p. 127-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Collagen fibrillar skeleton in pregnant rabbit endometrium at term
T2 - A SEM study after NaOH maceration
AU - Goranova, Vania
AU - Vizza, Enrico
AU - Correr, Silvia
AU - Heyn, Rosemarie
AU - Motta, Pietro M.
PY - 1996/5
Y1 - 1996/5
N2 - This paper describes the spatial organization of the collagen fibrillar skeleton in pregnant rabbit endometrium at term, employing an alkali/low temperature/maceration technique followed by scanning electron microscopic (SEM) observations. Parallel light microscopic (LM) and transmission electron microscopic (TEM) investigations were made to identify the location and possible changes in the endometrial collagen network. Two different types of NaOH maceration were applied, demonstrating separately: 1) the collagen structures (2N-NaOH maceration removes the cells and basal laminae); and 2) the cellular elements (6N-NaOH maceration removes the collagen fibrils). After 2N-NaOH maceration, the collagen network of the endometrium is seen in a superficial compartment around the glands and a deep compartment situated near the endometrial-myometrial junction. Significant changes are observed only in the superficial compartment. The luminal mucosal surface is characterized by numerous thin projections reducing the uterine cavity which, as a consequence, further appears very irregular and highly convoluted. The subepithelial collagen network is composed of densely packed fibrils with a woven course. It contains many tubular or channel-like invaginations (100- 150 μm in width and 200-300 μm in length) where endometrial glands are located. These invaginations, corresponding to the glandular impressions, are extremely dilated, enlarged and variable in shape. The collagen fibrils are arranged concentrically around the glandular orifices without forming bundles. At the bottom of the spaces between the mucosal projections, small fenestrations (4-8 μm in diameter each) are present. They form small groups of about 10-20 in number and are due to the endometrial blood capillaries. The deep compartment of the endometrial collagen network is little altered, preserving its general lamellar arrangement. The changes in the endometrial collagen skeleton are due to a variety of complex mechanical and hormonal stimuli affecting the uterus during pregnancy. These may be significant for correct implantation, placentation and delivery.
AB - This paper describes the spatial organization of the collagen fibrillar skeleton in pregnant rabbit endometrium at term, employing an alkali/low temperature/maceration technique followed by scanning electron microscopic (SEM) observations. Parallel light microscopic (LM) and transmission electron microscopic (TEM) investigations were made to identify the location and possible changes in the endometrial collagen network. Two different types of NaOH maceration were applied, demonstrating separately: 1) the collagen structures (2N-NaOH maceration removes the cells and basal laminae); and 2) the cellular elements (6N-NaOH maceration removes the collagen fibrils). After 2N-NaOH maceration, the collagen network of the endometrium is seen in a superficial compartment around the glands and a deep compartment situated near the endometrial-myometrial junction. Significant changes are observed only in the superficial compartment. The luminal mucosal surface is characterized by numerous thin projections reducing the uterine cavity which, as a consequence, further appears very irregular and highly convoluted. The subepithelial collagen network is composed of densely packed fibrils with a woven course. It contains many tubular or channel-like invaginations (100- 150 μm in width and 200-300 μm in length) where endometrial glands are located. These invaginations, corresponding to the glandular impressions, are extremely dilated, enlarged and variable in shape. The collagen fibrils are arranged concentrically around the glandular orifices without forming bundles. At the bottom of the spaces between the mucosal projections, small fenestrations (4-8 μm in diameter each) are present. They form small groups of about 10-20 in number and are due to the endometrial blood capillaries. The deep compartment of the endometrial collagen network is little altered, preserving its general lamellar arrangement. The changes in the endometrial collagen skeleton are due to a variety of complex mechanical and hormonal stimuli affecting the uterus during pregnancy. These may be significant for correct implantation, placentation and delivery.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029885527&partnerID=8YFLogxK
M3 - Article
C2 - 8790859
AN - SCOPUS:0029885527
VL - 59
SP - 127
EP - 135
JO - Archives of Histology and Cytology
JF - Archives of Histology and Cytology
SN - 0914-9465
IS - 2
ER -