Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

A. Mauro; A. Bertolotto; I. Germano; G. Giaccone; M. T. Giordana; A. Migheli; D. Schiffer

doi:10.1007/BF00679991

Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

A. Mauro, A. Bertolotto, I. Germano, G. Giaccone, M. T. Giordana, A. Migheli, D. Schiffer

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Abstract

The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

Original language	English
Pages (from-to)	157-163
Number of pages	7
Journal	Histochemistry
Volume	80
Issue number	2
DOIs	https://doi.org/10.1007/BF00679991
Publication status	Published - Mar 1984

ASJC Scopus subject areas

Anatomy

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title = "Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation",

abstract = "The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.",

author = "A. Mauro and A. Bertolotto and I. Germano and G. Giaccone and Giordana, {M. T.} and A. Migheli and D. Schiffer",

year = "1984",

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AU - Mauro, A.

AU - Bertolotto, A.

AU - Germano, I.

AU - Giaccone, G.

AU - Giordana, M. T.

AU - Migheli, A.

AU - Schiffer, D.

PY - 1984/3

Y1 - 1984/3

N2 - The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

AB - The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

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Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

Abstract

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