Abstract
The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.
| Original language | English |
|---|---|
| Pages (from-to) | 157-163 |
| Number of pages | 7 |
| Journal | Histochemistry |
| Volume | 80 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 1984 |
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ASJC Scopus subject areas
- Anatomy
Cite this
Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation. / Mauro, A.; Bertolotto, A.; Germano, I.; Giaccone, G.; Giordana, M. T.; Migheli, A.; Schiffer, D.
In: Histochemistry, Vol. 80, No. 2, 03.1984, p. 157-163.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation
AU - Mauro, A.
AU - Bertolotto, A.
AU - Germano, I.
AU - Giaccone, G.
AU - Giordana, M. T.
AU - Migheli, A.
AU - Schiffer, D.
PY - 1984/3
Y1 - 1984/3
N2 - The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.
AB - The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.
UR - http://www.scopus.com/inward/record.url?scp=0021235758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021235758&partnerID=8YFLogxK
U2 - 10.1007/BF00679991
DO - 10.1007/BF00679991
M3 - Article
C2 - 6325375
AN - SCOPUS:0021235758
VL - 80
SP - 157
EP - 163
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 2
ER -