Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

A. Mauro, A. Bertolotto, I. Germano, G. Giaccone, M. T. Giordana, A. Migheli, D. Schiffer

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalHistochemistry
Volume80
Issue number2
DOIs
Publication statusPublished - Mar 1984

ASJC Scopus subject areas

  • Anatomy

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