Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

A. Mauro, A. Bertolotto, I. Germano, G. Giaccone, M. T. Giordana, A. Migheli, D. Schiffer

Research output: Contribution to journalArticle

Abstract

The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalHistochemistry
Volume80
Issue number2
DOIs
Publication statusPublished - Mar 1984

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Tissue Fixation
Nerve Tissue
Factor VII
Pepsin A
Laminin
Collagenases
Fibronectins
Trypsin
Fixatives
Factor VIII
Formaldehyde
Digestion
Peptide Hydrolases

ASJC Scopus subject areas

  • Anatomy

Cite this

Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation. / Mauro, A.; Bertolotto, A.; Germano, I.; Giaccone, G.; Giordana, M. T.; Migheli, A.; Schiffer, D.

In: Histochemistry, Vol. 80, No. 2, 03.1984, p. 157-163.

Research output: Contribution to journalArticle

Mauro, A. ; Bertolotto, A. ; Germano, I. ; Giaccone, G. ; Giordana, M. T. ; Migheli, A. ; Schiffer, D. / Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation. In: Histochemistry. 1984 ; Vol. 80, No. 2. pp. 157-163.
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