Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood

Susanna Esposito, Claudio G. Molteni, Cristina Daleno, Antonia Valzano, Claudia Tagliabue, Carlotta Galeone, Gregorio Milani, Emilio Fossali, Paola Marchisio, Nicola Principi

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Abstract

This study evaluated the efficiency of pediatric mid-turbinate nasal flocked swabs used by parents in 203 children aged 6 months to 5 years with signs and symptoms of respiratory disease. Two nasal samples were collected from each child in a randomised sequence: one by a trained pediatrician and one by a parent. The real-time polymerase chain reaction influenza virus detection rates were similar in the samples collected using the two methods (Cohen's kappa=0.86), as were the cycle threshold values. In comparison with the pediatrician-collected samples, the sensitivity and specificity of the parental collections were respectively 89.3% (95% confidence interval [CI]: 77.8-100%) and 97.7% (95% CI: 95.5-100%), and the positive and negative predictive values were respectively 86.2% (95% CI: 73.7-95.1%) and 98.2% (95% CI: 96.4-100%). The children were significantly more satisfied with the parental collections (median values +/- standard deviation, 1.59 +/- 0.55 vs 3.51 +/- 0.36; p

Original languageEnglish
Pages (from-to)85
Number of pages1
JournalVirology Journal
DOIs
Publication statusAccepted/In press - Apr 30 2010

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ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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