Collection of 'normal' blood repopulating cells during early hemopoietic recovery after intensive conventional chemotherapy in chronic myelogenous leukemia

A. M. Carella, M. Podesta, F. Frassoni, M. R. Raffo, N. Pollicardo, E. Pungolino, R. Vimercati, M. Sessarego, C. Parodi, C. Rabitti, R. Ferrero, F. Benvenuto, O. Figari, P. Carlier, G. Levcasic, M. Valbonesi, V. Vitale, D. Giordano, D. Pierluigi

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Abstract

Twenty-five patients with CML (chronic phase (CP): 15 patients; accelerated phase (AP): 10 patients) at a median of 40 months after diagnosis and ineligible for allogeneic BMT, received an intensive chemotherapy regimen consisting of idarubicin, intermediate-dose ara-C and etoposide (ICE protocol). All patients had previously received alpha-interferon and only two patients had had partial cytogenetic response. During recovery from chemotherapy-induced aplasia, blood progenitors cells (BPC) were harvested by leukapheresis. All metaphases were found to be Ph-negative in the collection of 12 of 25 (48%) patients (CP: 9 of 15 (60%), AP: 3 of 10 (30%)) and a decrease of <50% Ph-positive metaphases was seen in an additional five (CP: 4 patients; AP: 1 patient). The percentage of complete Ph-disappearance was 66% in patients receiving this procedure within the first 2 years of diagnosis and 30% in those treated after the second year of diagnosis. So far, the Ph-negative collections have been used in 9 patients (CP: 8 patients; AP: 1 patient) as autograft after conditioning with total body irradiation/etoposide/CY. Seven of 9 patients engrafted and 5 are alive and well, Ph-negative at 2+, 3+, 6+, 10+ and 18+ months.

Original languageEnglish
Pages (from-to)267-271
Number of pages5
JournalBone Marrow Transplantation
Volume12
Issue number3
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Carella, A. M., Podesta, M., Frassoni, F., Raffo, M. R., Pollicardo, N., Pungolino, E., Vimercati, R., Sessarego, M., Parodi, C., Rabitti, C., Ferrero, R., Benvenuto, F., Figari, O., Carlier, P., Levcasic, G., Valbonesi, M., Vitale, V., Giordano, D., & Pierluigi, D. (1993). Collection of 'normal' blood repopulating cells during early hemopoietic recovery after intensive conventional chemotherapy in chronic myelogenous leukemia. Bone Marrow Transplantation, 12(3), 267-271.