Collection of peripheral blood hematopoietic progenitors (PBHP) from patients with severe aplastic anemia (SAA) after prolonged administration of granulocyte colony-stimulating factor

A. Bacigalupo, G. Piaggio, M. Podestá, M. T. Van Lint, M. Valbonesi, G. Lercari, P. G. Mori, M. Pasino, E. Franchini, L. Rivabella, O. Figari, G. Sogno, M. R. Raffo, A. M. Marmont

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Abstract

The aim of this study was to test whether prolonged administration of granulocyte colony-stimulating factor (G-CSF) would allow the collection by leukapheresis of PBHP in patients with SAA. For this purpose, nine SAA patients, 7 to 46 years old, six of whom were enrolled at diagnosis of their disease and three after previous immunosuppression had failed, were treated with antilymphocyte globulin (ALG) (day 1 to 5), cyclosporin A (5 mg/kg/d orally) (day 6 to 90) and G-CSF 5 μg/kg/d (day 6 to 90). A total of 40 leukaphereses were performed, (range 2 to 7 per patient), between days +10 and + 168 from G-CSF treatment. White blood cell count at the time of harvest ranged from 1.2 to 18.1 × 109/L. Results can be summarized as follows: the median number of cells collected per patient was 5.0 × 108/kg (range 2.6 to 18.7), the median number of CD34+ cells was 1.8 × 106/kg (range 0.27 to 3.8) and the median number of colony-forming units granulocyte-macrophage (CFU-GM) was 3.9 × 104/kg (range 0 to 39). Twenty leukaphereses performed between days + 33 and + 77 of G-CSF treatment grew granulocyte macrophages and erythroid colonies in vitro. No colony growth was obtained from 20 leukaphereses performed before day + 33 or after day + 80. In six patients the total number of CFU-GM recovered were in the range described for autologous peripheral blood stem cell grafts. (2.6 to 39 × 104/kg). In conclusion, this study suggests that circulating hematopoietic progenitors can be recovered after ALG priming and after at least 1 month of G-CSF treatment in a proportion of patients with SAA. Whether these cells will be suitable for autologous transplantation remains to be determined.

Original languageEnglish
Pages (from-to)1410-1414
Number of pages5
JournalBlood
Volume82
Issue number5
Publication statusPublished - Sep 1 1993

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Bacigalupo, A., Piaggio, G., Podestá, M., Van Lint, M. T., Valbonesi, M., Lercari, G., Mori, P. G., Pasino, M., Franchini, E., Rivabella, L., Figari, O., Sogno, G., Raffo, M. R., & Marmont, A. M. (1993). Collection of peripheral blood hematopoietic progenitors (PBHP) from patients with severe aplastic anemia (SAA) after prolonged administration of granulocyte colony-stimulating factor. Blood, 82(5), 1410-1414.