Raccolta di cellule staminali periferiche in pazienti di eta pediatrica: Valore predittivo della conta pre-leucaferesi delle cellule CD34+

Translated title of the contribution: Collection of peripheral blood stem cells in pediatric patients: Predictive value of the pre-leucapheresis number of CD34+ cells

R. Giordano, P. Marson, G. De Silvestro, C. Donadel, M. Vicarioto, M. G. Petris, C. Messina, R. Destro, M. V. Gazzola, L. Zanesco, G. Ongaro

Research output: Contribution to journalArticle

Abstract

Leucapheresis for peripheral blood stem cell collection (PBSCC) by continuous-flow blood cell separators, is largely used in adult patients undergoing high-dose chemotherapy for haematological malignancies and solid tumours. At present, this procedure is more and more used in paediatric oncohaematology. In this field, owing to the patient's features, it gives rise to some practical problems, among which the optimal tempo to have the best collection with the smallest number of leucaphereses. The aim of this study was to establish the predictive value of some basal parameters, such as white blood cells (WBC), mononuclear cells (MNC), and CD34+ cells on the leucapheresis yield. Forty-six patients, aged between I and 18 years, affected by various haematological and solid malignancies, underwent seventy- four leucaphereses, after mobilizing chemotherapy plus G-CSF. Among all the parameters we have analysed, only the pre-leucapheresis number of CD34+ cells (absolute count or percent) has been found to be strongly associated with the yield of the CD34+ cell collections. In addiction, a basal value of almost 45 CD34+ cells/μL was predictive of a final amount of ≥4x106/Kg CD34+ cells, in a single leucapheresis, processing about 2,5 blood volumes (χ2=50.27, p

Translated title of the contributionCollection of peripheral blood stem cells in pediatric patients: Predictive value of the pre-leucapheresis number of CD34+ cells
Original languageItalian
Pages (from-to)304-312
Number of pages9
JournalTrasfusione del Sangue
Volume44
Issue number6
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

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