Colony-stimulating factor produced by murine adrenocortical tumor cells

A. Pessina, M. G. Neri, A. Muschiato, P. Brambilla, A. Marocchi, P. Mocarelli

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Conditioned medium obtained from the adrenocortical LAF1 mouse tumor (Y-1) cell cultures was able to stimulate the proliferation and the differentiation of granulocyte-macrophage precursors in the normal murine bone marrow. Colony-stimulating factor (CSF) was spontaneously produced by Y-1 cells also in serum-free cultures. By two cycles of gel chromatography on Sephadex G-150 of concentrated conditioned medium two peaks of colony-stimulating activity were isolated that corresponded to apparent molecular radii of 100,000 and 29,000, respectively. At this step of purification, the two factors gave a similar dose-response curve, showed a remarkable resistance to the heat treatment and pH changes, and were not extracted by ether. Because Y-1 cells resulted in infection by retrovirus, they provide a useful model to investigate the relations between viral coded information and CSF production.

Original languageEnglish
Pages (from-to)1095-1099
Number of pages5
JournalJournal of the National Cancer Institute
Issue number6
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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