Colostral t lymphocytes detected by intracytoplasmic and membrane markers

C. Pignata; L. Nespol; R. Maccario; S. Siena; G. M Ingrat; M. Mazzucco; A. G. Ugazio

doi:10.1159/000241602

Colostral t lymphocytes detected by intracytoplasmic and membrane markers

C. Pignata, L. Nespol, R. Maccario, S. Siena, G. M Ingrat, M. Mazzucco, A. G. Ugazio

Research output: Contribution to journal › Article › peer-review

Abstract

Colostral lymphocytes were studied using two established T-cell markers: Intracytoplasmic alpha-naphtyl-acetate esterase (ANAE) staining and membrane receptors for sheep erythrocytes (E rosettes). ANAE staining allowed counting and identification of T-cell subsets independently of the status of membrane structures and receptors frequently altered in colostral cells. The fact that a sizeable number of colostral lymphocytes had the same phenotype as the majority of mature circulating peripheral blood lymphocytes supports the hypothesis that colostral lymphocytes may play a role in protecting neonates against infections, in transferring immune information to the newborn, or in modulating the immune response via release of soluble factors. A considerable percentage of colostral T lymphocytes are ANAE-negative. This phenotype is similar to that observed among thymocytes.

Original language	English
Pages (from-to)	217-221
Number of pages	5
Journal	Neonatology
Volume	42
Issue number	5-6
DOIs	https://doi.org/10.1159/000241602
Publication status	Published - 1982

Keywords

Colostrum
Neonatal immunology
Newborn
T lymphocytes

ASJC Scopus subject areas

Developmental Biology
Pediatrics, Perinatology, and Child Health

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title = "Colostral t lymphocytes detected by intracytoplasmic and membrane markers",

abstract = "Colostral lymphocytes were studied using two established T-cell markers: Intracytoplasmic alpha-naphtyl-acetate esterase (ANAE) staining and membrane receptors for sheep erythrocytes (E rosettes). ANAE staining allowed counting and identification of T-cell subsets independently of the status of membrane structures and receptors frequently altered in colostral cells. The fact that a sizeable number of colostral lymphocytes had the same phenotype as the majority of mature circulating peripheral blood lymphocytes supports the hypothesis that colostral lymphocytes may play a role in protecting neonates against infections, in transferring immune information to the newborn, or in modulating the immune response via release of soluble factors. A considerable percentage of colostral T lymphocytes are ANAE-negative. This phenotype is similar to that observed among thymocytes.",

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T1 - Colostral t lymphocytes detected by intracytoplasmic and membrane markers

AU - Pignata, C.

AU - Nespol, L.

AU - Maccario, R.

AU - Siena, S.

AU - M Ingrat, G.

AU - Mazzucco, M.

AU - Ugazio, A. G.

PY - 1982

Y1 - 1982

N2 - Colostral lymphocytes were studied using two established T-cell markers: Intracytoplasmic alpha-naphtyl-acetate esterase (ANAE) staining and membrane receptors for sheep erythrocytes (E rosettes). ANAE staining allowed counting and identification of T-cell subsets independently of the status of membrane structures and receptors frequently altered in colostral cells. The fact that a sizeable number of colostral lymphocytes had the same phenotype as the majority of mature circulating peripheral blood lymphocytes supports the hypothesis that colostral lymphocytes may play a role in protecting neonates against infections, in transferring immune information to the newborn, or in modulating the immune response via release of soluble factors. A considerable percentage of colostral T lymphocytes are ANAE-negative. This phenotype is similar to that observed among thymocytes.

AB - Colostral lymphocytes were studied using two established T-cell markers: Intracytoplasmic alpha-naphtyl-acetate esterase (ANAE) staining and membrane receptors for sheep erythrocytes (E rosettes). ANAE staining allowed counting and identification of T-cell subsets independently of the status of membrane structures and receptors frequently altered in colostral cells. The fact that a sizeable number of colostral lymphocytes had the same phenotype as the majority of mature circulating peripheral blood lymphocytes supports the hypothesis that colostral lymphocytes may play a role in protecting neonates against infections, in transferring immune information to the newborn, or in modulating the immune response via release of soluble factors. A considerable percentage of colostral T lymphocytes are ANAE-negative. This phenotype is similar to that observed among thymocytes.

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KW - Neonatal immunology

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Colostral t lymphocytes detected by intracytoplasmic and membrane markers

Abstract

Keywords

ASJC Scopus subject areas

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