Abstract
Reduction of the window period of hepatitis C virus (HCV) infection represents an important goal in the transfusional and diagnostic setting. A prototype assay designed to simultaneously detect circulating HCV antigen and anti-HCV, has been developed. Aim of this study was to evaluate the performance of this new assay in terms of specificity and sensitivity and to compare its efficacy with commercial assays. To evaluate the specificity of the assay, 400 samples from the general population and 100 'difficult' sera, negative for anti-HCV, were tested. To assess sensitivity, the new test was used on 76 PCR-positive and anti-HCV negative sera, seven natural or commercial seroconversion panels that included 17 RNA-positive and anti-HCV negative sera and 31 anti-HCV positive sera, 20 weak anti-HCV positive sera, 80 viraemic and anti-HCV-positive sera from patients infected with different subtypes and 10 sera from patients with HBV-HCV or HIV-HCV co-infections. Of 500 anti-HCV negative samples, 499 (99.8%) were negative with a cut-off index
| Original language | English |
|---|---|
| Pages (from-to) | 5-10 |
| Number of pages | 6 |
| Journal | Journal of Viral Hepatitis |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2006 |
Fingerprint
Keywords
- Antigen
- Diagnosis
- HCV
- Immunoassay
- Window period
ASJC Scopus subject areas
- Hepatology
- Virology
Cite this
Combination hepatitis C virus antigen and antibody immunoassay as a new tool for early diagnosis of infection. / Ansaldi, F.; Bruzzone, B.; Testino, G.; Bassetti, M.; Gasparini, R.; Crovari, P.; Icardi, G.
In: Journal of Viral Hepatitis, Vol. 13, No. 1, 01.2006, p. 5-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Combination hepatitis C virus antigen and antibody immunoassay as a new tool for early diagnosis of infection
AU - Ansaldi, F.
AU - Bruzzone, B.
AU - Testino, G.
AU - Bassetti, M.
AU - Gasparini, R.
AU - Crovari, P.
AU - Icardi, G.
PY - 2006/1
Y1 - 2006/1
N2 - Reduction of the window period of hepatitis C virus (HCV) infection represents an important goal in the transfusional and diagnostic setting. A prototype assay designed to simultaneously detect circulating HCV antigen and anti-HCV, has been developed. Aim of this study was to evaluate the performance of this new assay in terms of specificity and sensitivity and to compare its efficacy with commercial assays. To evaluate the specificity of the assay, 400 samples from the general population and 100 'difficult' sera, negative for anti-HCV, were tested. To assess sensitivity, the new test was used on 76 PCR-positive and anti-HCV negative sera, seven natural or commercial seroconversion panels that included 17 RNA-positive and anti-HCV negative sera and 31 anti-HCV positive sera, 20 weak anti-HCV positive sera, 80 viraemic and anti-HCV-positive sera from patients infected with different subtypes and 10 sera from patients with HBV-HCV or HIV-HCV co-infections. Of 500 anti-HCV negative samples, 499 (99.8%) were negative with a cut-off index
AB - Reduction of the window period of hepatitis C virus (HCV) infection represents an important goal in the transfusional and diagnostic setting. A prototype assay designed to simultaneously detect circulating HCV antigen and anti-HCV, has been developed. Aim of this study was to evaluate the performance of this new assay in terms of specificity and sensitivity and to compare its efficacy with commercial assays. To evaluate the specificity of the assay, 400 samples from the general population and 100 'difficult' sera, negative for anti-HCV, were tested. To assess sensitivity, the new test was used on 76 PCR-positive and anti-HCV negative sera, seven natural or commercial seroconversion panels that included 17 RNA-positive and anti-HCV negative sera and 31 anti-HCV positive sera, 20 weak anti-HCV positive sera, 80 viraemic and anti-HCV-positive sera from patients infected with different subtypes and 10 sera from patients with HBV-HCV or HIV-HCV co-infections. Of 500 anti-HCV negative samples, 499 (99.8%) were negative with a cut-off index
KW - Antigen
KW - Diagnosis
KW - HCV
KW - Immunoassay
KW - Window period
UR - http://www.scopus.com/inward/record.url?scp=33644874357&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644874357&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2893.2005.00646.x
DO - 10.1111/j.1365-2893.2005.00646.x
M3 - Article
C2 - 16364076
AN - SCOPUS:33644874357
VL - 13
SP - 5
EP - 10
JO - Journal of Viral Hepatitis
JF - Journal of Viral Hepatitis
SN - 1352-0504
IS - 1
ER -