TY - JOUR
T1 - Combined analysis of miR-200 family and its significance for breast cancer
AU - Fontana, Andrea
AU - Barbano, Raffaela
AU - Dama, Elisa
AU - Pasculli, Barbara
AU - Rendina, Michelina
AU - Morritti, Maria Grazia
AU - Melocchi, Valentina
AU - Castelvetere, Marina
AU - Valori, Vanna Maria
AU - Ravaioli, Sara
AU - Bravaccini, Sara
AU - Ciuffreda, Luigi
AU - Graziano, Paolo
AU - Maiello, Evaristo
AU - Copetti, Massimiliano
AU - Fazio, Vito Michele
AU - Esteller, Manel
AU - Bianchi, Fabrizio
AU - Parrella, Paola
N1 - Funding Information:
This work was supported by Italian Ministry of Health (MoH) TRANSCAN Joint Transnational Call (JTC) 2013 co-funded by the European Regional Development Fund, “A way of making Europe” RRC-2014-2354565, Italian Ministry of Health (MoH) “Ricerca Corrente 2019” and “5×1000″ voluntary contributions” and partially supported by Associazione Italiana Ricerca sul Cancro (AIRC) Project Code: 16747.
Publisher Copyright:
© 2021, The Author(s).
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/12
Y1 - 2021/12
N2 - While the molecular functions of miR-200 family have been deeply investigated, a role for these miRNAs as breast cancer biomarkers remains largely unexplored. In the attempt to clarify this, we profiled the miR-200 family members expression in a large cohort of breast cancer cases with a long follow-up (H-CSS cohort) and in TCGA-BRCA cohort. Overall, miR-200 family was found upregulated in breast tumors with respect to normal breast tissues while downregulated in more aggressive breast cancer molecular subtypes (i.e. Luminal B, HER2 and triple negative), consistently with their function as repressors of the epithelial-to-mesenchymal transition (EMT). In particular miR-141-3p was found differentially expressed in breast cancer molecular subtypes in both H-CSS and TCGA-BRCA cohorts, and the combined analysis of all miR-200 family members demonstrated a slight predictive accuracy on H-CSS cancer specific survival at 12 years (survival c-statistic: 0.646; 95%CI 0.538–0.754).
AB - While the molecular functions of miR-200 family have been deeply investigated, a role for these miRNAs as breast cancer biomarkers remains largely unexplored. In the attempt to clarify this, we profiled the miR-200 family members expression in a large cohort of breast cancer cases with a long follow-up (H-CSS cohort) and in TCGA-BRCA cohort. Overall, miR-200 family was found upregulated in breast tumors with respect to normal breast tissues while downregulated in more aggressive breast cancer molecular subtypes (i.e. Luminal B, HER2 and triple negative), consistently with their function as repressors of the epithelial-to-mesenchymal transition (EMT). In particular miR-141-3p was found differentially expressed in breast cancer molecular subtypes in both H-CSS and TCGA-BRCA cohorts, and the combined analysis of all miR-200 family members demonstrated a slight predictive accuracy on H-CSS cancer specific survival at 12 years (survival c-statistic: 0.646; 95%CI 0.538–0.754).
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U2 - 10.1038/s41598-021-82286-1
DO - 10.1038/s41598-021-82286-1
M3 - Article
AN - SCOPUS:85100382164
VL - 11
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 2980
ER -